| CleanAmp™-Pac-dA-CE Phosphoramidite |
Catalog Number: 10-1440-xx
Description: CleanAmp™-Pac-dA-CE Phosphoramidite
5'-Dimethoxytrityl-N-phenoxyacetyl-2'-deoxyAdenosine,
3'-[(4-oxo-tetradecyl)-(N,N-diisopropyl)]-phosphoramidite |
| Formula: C59H77N6O9P |
M.W.: 1045.25 |
F.W.: FW: 523.56 (triester) |
Diluent: Anhydrous Acetonitrile |
| Coupling: 10 minute coupling time. CleanAmp™ monomers must be used with UltraMild phosphoramidites and Cap Mix. |
| Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature. See Glen Report 21.2 for details. (www.glenresearch.com/GlenReports/GR21-2.pdf) |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 24 hours |
CleanAmp™ Primers
CleanAmp™ Primers offer an alternative to other Hot Start technologies and allow greater control of primer hybridization and extension during PCR. It has been demonstrated that CleanAmp™ Primers outperform other technologies in multiple applications. Indeed, over a broad range of applications, CleanAmp™ Primers reduce or eliminate off-target amplification. Greater amplicon yield is also achieved, due to improvement in specificity and sensitivity. By using either the slow-releasing Precision primers with two CleanAmp™ phosphotriester linkages or the faster-releasing Turbo Primers with a single CleanAmp™ phosphotriester linkage, the rate of formation of unmodified primer can be controlled to suit reaction needs. A table to aid in the selection of Turbo and Precision Primers for specific applications is shown below.
| Turbo Primers |
Precision Primers |
| Fast cycling |
Standard cycling |
| Multiplex PCR |
One-step reverse-transcription PCR |
| Improves amplicon yield |
Improved specificity and limit of detection |
| Reduces mis-priming/ primer dimer formation |
Greatest reduction in mis-priming/primer dimer formation |
Synthesis of CleanAmp™ Primers requires the use of UltraMild Chemistry.
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