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Oligo Synthesis

Oligo Synthesis : CEPs

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2-Thio-dT-CE Phosphoramidite

2-Thio-dT-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1036-022-Thio-dT-CE Phosphoramidite 0.25g £540.00£513.00Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-1036-902-Thio-dT-CE Phosphoramidite 100µmoles £236.00£224.20Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-1036-952-Thio-dT-CE Phosphoramidite 50µmoles £132.00£125.40Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order

2-Thio-dT-CE Phosphoramidite

2-Thio-dT-CE Phosphoramidite

Glen Research

2-Thio-dT-CE Phosphoramidite

Structure

Catalog Number: 10-1036-xx

Description: 2-Thio-dT-CE Phosphoramidite

5'-Dimethoxytrityl-(N3/O4-toluoyl)-2'-deoxy-2-thioThymidine,
3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C48H55N4O8PS M.W.: 879.02 F.W.: 320.26

Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. Technical Bulletin
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 2-3 days

The C-nucleoside 2’-deoxypseudouridine, in contrast to dU, forms stable C:pseudoU-A triplets. 2-Aminopurine lacks groups critical for hydrogen bonding and is a mildly fluorescent base.

Demand for sulfur modified bases continues to expand for investigations of oligonucleotide structure, but primarily for cross-linking purposes. 6-Thio-dG, 4-Thio-dT and 4-thio-dU are very useful modifications for photo cross-linking and photoaffinity labelling experiments. Oligos containing 2-thio-dT are useful in examining protein-DNA interaction by acting as photosensitizing probes. The thiocarbonyl group in 2-thio-dT is especially interesting in that it is available to react with compounds associating with the minor groove of DNA. 2-Amino-A forms a very stable base pair with T containing three hydrogen bonds but the stability of the base pair with 2-thio-T is greatly diminished. Due to steric interactions between the 2-thio group of thymidine and the 2-amino group of 2-amino-A, the base pair contains only a single hydrogen bond. Oligos containing 2-amino-dA and 2-thio-dT exhibit high affinity for natural oligonucleotides but show little affinity for other similar oligos even of a complementary sequence.

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2-Thio-dT-CE Phosphoramidite

2-Thio-dT-CE Phosphoramidite

Glen Research

 

MSDS

Glen Report 8.2

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2-Thio-dT-CE Phosphoramidite

2-Thio-dT-CE Phosphoramidite

Glen Research

EXTINCTION DATA

Item Nucleoside λMax-1 Emax-1 λMax-2 Emax-2 E260


(nm) (ml/µmole) nm (ml/µmole) (ml/µmole)
10-1036 2-thio-dT 278 17.5 220 14.8 10.0

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1036-02 0.25grams .25grams 2.84 81.33 48.8 30.5 22.18 16.27 4.07
10-1036-90 100µmoles .088grams 1 20 12 7.5 5.45 4 1
10-1036-95 50µmoles .044grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1036-02 0.25grams .25grams 4.24 .07 78.4 49 35.64 4.9
10-1036-90 100µmoles .088grams 1.5 .07 23.6 14.75 10.73 1.48
10-1036-95 50µmoles .044grams .75 .07 8.6 5.38 3.91 .54
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1036-02 0.25grams .25grams 4.24 .07 80 50 36.36

10-1036-90 100µmoles .088grams 1.5 .07 25.2 15.75 11.45

10-1036-95 50µmoles .044grams .75 .07 10.2 6.38 4.64

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