- Produce micrograms of biotin-RNA or biotin-aRNA
- Save time with 30 minute high-yield protocol, or extend the incubation to increase yield even further
Applications
- Production of nonradioactive RNA probes for any purpose, including microarray (transcription profiling) studies, in situ hybridization, and blotting experiments.
The AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit enables high-yield synthesis of biotin-RNA or biotin-antisense RNA (aRNA, also called cRNA). The kit incorporates Biotin-16-UTP directly into the RNA transcript. High yields of biotin-RNA are produced from as little as 50 ng of input DNA template.
Features
- Exceptionally high yields. The kit uses Epicentre's AmpliScribe T7-Flash rapid, high-yield in vitro transcription technology to generate the highest possible yield of biotin-RNA.
- Optimized. In vitro transcription reaction conditions maximize yield of uniformly labelled biotin-RNA for high signal intensity.
- Convenient. Rnase Inhibitor included.
- Convenient. Rnase Inhibitor included.
| |
|
5 - 10 µg
|
15 - 25 µg
|
30 - 40 µg
|
60 - 70 µg
|
|
15 - 20 µg
|
35 - 45 µg
|
70 - 80 µg
|
130 - 140 µg
|
|
60 - 70 µg
|
140 - 150 µg
|
> 180 µg
|
> 180 µg
|
|
125 - 135 µg
|
> 180 µg
|
> 180 µg
|
> 180 µg
|
|
|
Table 1. Yield of Biotin-RNA (in mg) from varying amounts of control template DNA from a standard 37°C, 20 ml AmpliScribe T7-Flash Biotin-RNA Transcription Kit reaction over time. Results may vary depending on the template used.
.
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200