Ribonuclease A (RNase A) is an endoribonuclease that cleaves single-stranded RNA at the 3'-end of pyrimidine residues, forming oligoribonucleotides having 3'-terminal pyrimidine-3'-phosphates. Pyrimidine-3'-monophosphates are also released by RNase A cleavage of adjacent pyrimidine nucleotides.
Modified RNA containing pyrimidine-2'-fluoro-dNMPs, such as modified RNA made by in vitro transcription using the DuraScribe® T7 & SP6 Transcription Kits is completely resistant to cleavage by RNase A.
Composition and Concentration
Provided on the basis of weight at 5 mg/ml in Storage Buffer
50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DDT, 0.1 M NaCl, and 0.1% Triton® X-100
RNase A is free of contaminating DNA exo- and endonuclease activities
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