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Lab Reagents

Lab Reagents: Absolute Lectins

Absolute Lectins, perfect for Glycoscience Medicago is a primary manufacturer of a wide variety of exceptionally pure lectins purified by affinity chromatography. Lectins are non-enzymatic proteins of non-immunoglobulin origin that bind specifically and reversibly to carbohydrate moieties without altering the covalent structure of the glycosyl ligands. The term ‘lectin’ is derived from the latin word legere, meaning ‘to select’. The specificity of a lectin is usually defined by the monosaccharides or oligosaccharides that are best at inhibiting the agglutination or precipitation caused by the lectin. Lectins are usually of plant origin but do occur in many types of organism; some are glycoproteins and may be soluble or membrane-bound. The biological function of lectins is far from fully explored. Their specificity enables binding to glycoproteins and polysaccharides as well as agglutination of erythrocytes and stimulation of blood lymphocytes. Because of their ability to distinguish glycosyl ligands on human red blood cells, lectins can be used for blood typing.Immobilized lectins can be used in affinity chromatography to purify and isolate glycoproteins, glycolipids, polysaccharides, viruses and cells. Carbohydrate-containing substances bound to the lectin may be eluted with a competitive binding substance. Medicago offers lectins as a freeze-dried powder or as a solution manufactured under aseptic conditions. If you don’t find the lectin you are looking for, we can certainly produce it for you. Furthermore, we offer different custom lectin conjugates on request. •Ultrapure quality •High activity •Wide range •Lyophilized powder •Custom lectins and conjugates

Phaseolus vulgaris lectin M (PHA-M)

PHA-M is the mucoprotein variant of phytohemaglutinin isolated from red kidney bean. It contains up to 20% carbohydrates in conjugation with the protein which presents two bands corresponding to about 35 kDa according to SDS-PAGE in presence of mercaptoethanol. It is a potent mitogen used to stimulate cell proliferation in lymphocyte cultures and also has a powerful erythroagglutinating activity.

Medicago AB

Catalogue No.DescriptionPack SizePriceQty
MC-05-0118-1000Phaseolus vulgaris lectin M (PHA-M), crude 1000mg POA Quantity Add to Order
MC-05-0118-25Phaseolus vulgaris lectin M (PHA-M), crude 25mg £95.00 Quantity Add to Order
MC-05-1118-1000Phaseolus vulgaris lectin M (PHA-M), pure1000mg POA Quantity Add to Order
MC-05-1118-25Phaseolus vulgaris lectin M (PHA-M), pure25mg £150.00 Quantity Add to Order

Phaseolus vulgaris lectin M (PHA-M)

PHA-M is the mucoprotein variant of phytohemaglutinin isolated from red kidney bean. It contains up to 20% carbohydrates in conjugation with the protein which presents two bands corresponding to about 35 kDa according to SDS-PAGE in presence of mercaptoethanol. It is a potent mitogen used to stimulate cell proliferation in lymphocyte cultures and also has a powerful erythroagglutinating activity.

Medicago AB

Features

• Two grades of purity; PHA-M pure and PHA-M crude
• Potent mitogen
• High erythroagglutinating activity

Phaseolus vulgaris lectin M (PHA-M)

Applications

• Stimulation of cell proliferation in lymphocyte cultures • Erythroagglutination

 

Description

PHA-M is the mucoprotein variant of phytohemaglutinin isolated from red kidney bean. It contains up to 20% carbohydrates in conjugation with the protein which presents two bands corresponding to about 35 kDa according to SDS-PAGE in presence of mercaptoethanol. It is a potent mitogen used to stimulate cell proliferation in lymphocyte cultures and also has a powerful erythroagglutinating activity.

Storage and stability

Stable for at least 3 years from production date when stored below -20 °C. May be shipped at ambient temperature. After reconstitution in PBS pH 7.4, the solution may be stored frozen in working aliquots for up to 12 months. Repeated freez- ing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

References

(1) Liener I. E., Sharon N., Goldstein I. J., (1986) The Lectins – Properties, Functions and Applications in Biology and Medicine.

MSDS: 
application/pdf iconMSDS PHA-M

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