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PCR : PCR Products

The FailSafe™ PCR system for templates up to 20kb long, high GC content (up to 85% G+C), & Multiplex PCR

Sickle Cell Genetics Lab- Diagnosing Baby Marie

Learning lab : In this DNA gel electrophoresis investigation, students are presented with a fictional family’s medical history and must work to make a genetic diagnosis for possible sickle cell disease.
Techniques utilized: DNA gel electrophoresis.
Time required: can be completed in one 45 min. class period
Reagents needed: ‘Sickle Cell Genetics Lab’ reagents kit (included in this product), electrophoresis reagents (available separately)

MiniPCR - Amplyus

Catalogue No.DescriptionPack SizePriceQty
MP-KT-1502-01Sickle Cell Genetics Lab: Diagnosing Baby Marie each £70.00 Quantity Add to Order

Sickle Cell Genetics Lab- Diagnosing Baby Marie

Learning lab : In this DNA gel electrophoresis investigation, students are presented with a fictional family’s medical history and must work to make a genetic diagnosis for possible sickle cell disease.
Techniques utilized: DNA gel electrophoresis.
Time required: can be completed in one 45 min. class period
Reagents needed: ‘Sickle Cell Genetics Lab’ reagents kit (included in this product), electrophoresis reagents (available separately)

MiniPCR - Amplyus

Description

Students are presented with a fictional family’s medical history and must work to make a genetic diagnosis. The family depicted in this lab, the Robinson family, has two children, one of whom has had an initial test result indicating possible sickle cell disease.

This hands-on lab activity exposes students to the fundamental principles of DNA analysis comparing patterns of cleaved DNA by gel electrophoresis. Students will gain familiarity with DNA separation techniques and engage in determining DNA fragment size to obtain a genetic diagnosis.

  • Techniques utilized: DNA gel electrophoresis.
  • Time required: can be completed in one 45 min. class period
  • Reagents needed: ‘Sickle Cell Genetics Lab’ reagents kit (included in this product), electrophoresis reagents (available separately)

Suggested skill level: Intended for any student seeking familiarity with DNA gel electrophoresis, genetic disease and diagnosis, from middle school to college.

The Sickle Cell Genetics Lab kit contains reagents for 8 lab groups of up to 5 students each (40 students):

  • 4 DNA samples for fingerprinting analysis
  • 1 tube of DNA molecular weight marker (DNA Ladder)
  • Reagents are compatible with blueGel™ or other standard DNA electrophoresis systems

Please note:

Free miniPCR Learning Lab downloads:

Contact team@minipcr.com for answers key.

 

 

 

 

Optional: Lab Companion Kit

Microtubes (50), PCR tubes (100), blueGel™ Tabs agarose tablets (10 pcs. x 0.4 g ea.), 20X TBE buffer (30 mL), GelGreen™ DNA stain, 10,000X (20 µL). NOTE: this add-on item ships UPS GROUND at no additional charge. contact us for details

 

 

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