Applications

PCR : PCR Products

The FailSafe™ PCR system for templates up to 20kb long, high GC content (up to 85% G+C), & Multiplex PCR

MegaTaq polymerase

For use in polymerase chain reaction

Cambio

Description

MegaTaq Polymerase

(Data sheet available here)

Description

Highly purified, recombinant protein of approximately 94 kDa from Thermus aquaticus
An excellent and affordable Taq polymerase that comes with 10X PCR buffers in a ready-to-use format (either with or without electrophoresis tracking dyes) or with a buffer that lacks MgCl2 that allows researchers to test and/or use their own optimal magnesium ion concentration for primers and template of choice.

Applications

Recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 10 Kb
Optimal amplification of DNA at 74°C with a half-life of 40 minutes at 95°C
Taq DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5'→3' direction in the presence of magnesium ions
Exhibits 5'→3' exonuclease activity
Lacks 3'→5' exonuclease activity

Reagents Supplied

10X Taq PCR Buffer A
10X Taq PCR Buffer B
10X Taq PCR Buffer C

10X SuperTaq Buffer (original formulation available on request)

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmoles of dNTP into acid-insoluble material in 30 minutes at 70°C

10X Reaction Buffer

10X Taq Buffer A (optimization buffer without MgCl₂): Buffer allows to optimize MgCl₂concentration
10X Taq Buffer B (general application, up to 10 kb): Buffer contains 15 mM MgCl₂and is optimized for use with 0.2 mM of each dNTP
10X Taq Buffer C (contains coloured dyes): 10X Taq Buffer B contains two gel tracking dyes together with and a gel loading reagent; thus enabling the direct loading of PCR products onto an agarose gel without any further sample preparation.

Storage Buffer

20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol, stabilisers.

Quality Control

Assayed to confirm extremely minimal contaminating endonuclease, 3'-exonuclease, and nonspecific single- and double-stranded DNase activities.

Typical preparations are assessed to be of greater than 95% purity, as judged by SDS polyacrylamide gel electrophoresis.