Digest proteins during DNA and RNA purification or inactivate enzymes
Robust: Proteinase K is a highly stable protease that is active in most buffers including those with SDS and urea
Flexible: Use in applications outside of the MasterPure™ Kits including isolation of plasmids and high molecular weight gDNA, purification of intact RNA, and inactivation of Rnases, Dnases and other enzymes in molecular biology reactions
Proteinase K is a serine protease active against a wide variety of proteins. Proteinase K is stable and active in SDS and urea and is useful for a variety of applications such as purification of nuclease-free plasmids, high molecular weight genomic DNA and intact RNA.
- Supplied in solution at 50 µg/µL
- Functionally tested for purification of high molecular weight genomic DNA and intact RNA
- Active Temperature Range: Room temperature to 60°C
- Active pH Range: pH 4 to 12
- Calcium: 1 – 5 mM Ca 2
- Active under denaturing conditions such as 0.5% – 1% SDS or 1 M – 5 M urea
Storage and Testing
Storage Buffer: 50% glycerol, 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1mM EDTA, 10 mM CaCl2, 0.1% Triton X-100 and 1 mM DTT
Stability: Guaranteed to retain full activity for 12 months from date of purchase when stored at -20°C in the original container.
Function Tested: Proteinase K is function tested in purifying high molecular weight DNA or RNA using the Epicentre MasterPure™ DNA and RNA Purification Kit.
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