Cambio - Excellence in Molecular Biology

Extraction & Purification - DNA

Extraction & Purification - DNA: Total DNA

Fast and easy methods for the micro scale purification of total (genomic and mitochondrial) DNA from various biological samples.

Proteinase K

Digest proteins during DNA and RNA purification or inactivate enzymes Robust: Proteinase K is a highly stable protease that is active in most buffers including those with SDS and urea Flexible: Use in applications outside of the MasterPure™ Kits including isolation of plasmids and high molecular weight gDNA, purification of intact RNA, and inactivation of Rnases, Dnases and other enzymes in molecular biology reactions

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
Proteinase KProteinase K2ml £118.00 Quantity Add to Order

Proteinase K

Digest proteins during DNA and RNA purification or inactivate enzymes Robust: Proteinase K is a highly stable protease that is active in most buffers including those with SDS and urea Flexible: Use in applications outside of the MasterPure™ Kits including isolation of plasmids and high molecular weight gDNA, purification of intact RNA, and inactivation of Rnases, Dnases and other enzymes in molecular biology reactions

BioSearch Tech (Lucigen/Epicentre)

Proteinase K

Proteinase K is a serine protease active against a wide variety of proteins. Proteinase K is stable and active in SDS and urea and is useful for a variety of applications such as purification of nuclease-free plasmids, high molecular weight genomic DNA and intact RNA.

Characteristics

  • Supplied in solution at 50 µg/µL
  • Functionally tested for purification of high molecular weight genomic DNA and intact RNA
  • Active Temperature Range: Room temperature to 60°C
  • Active pH Range: pH 4 to 12
  • Calcium: 1 – 5 mM Ca 2
  • Active under denaturing conditions such as 0.5% – 1% SDS or 1 M – 5 M urea

Storage and Testing

Storage Buffer: 50% glycerol, 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1mM EDTA, 10 mM CaCl2, 0.1% Triton X-100 and 1 mM DTT
Store: -20°C
Stability: Guaranteed to retain full activity for 12 months from date of purchase when stored at -20°C in the original container.
Function Tested: Proteinase K is function tested in purifying high molecular weight DNA or RNA using the Epicentre MasterPure™ DNA and RNA Purification Kit.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Proteinase K

Digest proteins during DNA and RNA purification or inactivate enzymes Robust: Proteinase K is a highly stable protease that is active in most buffers including those with SDS and urea Flexible: Use in applications outside of the MasterPure™ Kits including isolation of plasmids and high molecular weight gDNA, purification of intact RNA, and inactivation of Rnases, Dnases and other enzymes in molecular biology reactions

BioSearch Tech (Lucigen/Epicentre)

Protocol

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200