Cambio - Excellence in Molecular Biology

Transfection-EZ Biosystems

Transfection-EZ Biosystems: Cell Lines

Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:

Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines). Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated. 

For primary cells click HERE

To learn why you should use Avalanche® Cell Type-Specific Transfection reagents, click HERE; To learn how EZ Biosystems developed Avalanche® Cell Type-Specific Transection Reagents, click HERE

HT-29 Cell Avalanche™ Transfection Reagent (human colon adenocarcinoma cell)

HT-29 Cell Avalanche™ Transfection Reagent (human colon adenocarcinoma cell)

EZ Biosystems

Catalogue No.DescriptionPack SizePriceQty
EZT-HT29-1-05HT-29 Cell Avalanche™ Transfection Reagent0.5ml £399.00 Quantity Add to Order
EZT-HT29-1-15HT-29 Cell Avalanche™ Transfection Reagent1.5ml £699.00 Quantity Add to Order

HT-29 Cell Avalanche™ Transfection Reagent (human colon adenocarcinoma cell)

HT-29 Cell Avalanche™ Transfection Reagent (human colon adenocarcinoma cell)

EZ Biosystems

Cell Line Information:

Designations: HT-29
Depositors: J Fogh
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: colon
Disease: colorectal adenocarcinoma
Isolation date: 1964
Applications: transfection host
Receptors: human adrenergic alpha2A [23560], urokinase receptor (u-PAR), vitamin D (moderate expression), vitamin D (moderate expression).
Tumorigenic: Yes
Age: 44 years adult
Gender: female
Ethnicity: Caucasian
Comments: Ultrastructural features reported for HT-29 cells include microvilli, microfilaments, large vacuolated mitochondria with dark granules, smooth and rough endoplasmic reticulum with free ribosomes. HT-29 cells are negative for CD4, but there is cell surface expression of galactose ceramide (a possible alternative receptor for HIV). The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes.

HT-29 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

The Transfection Reagent:

HT-29 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting HT-29 Cells. The proprietary formulation of lipids and polymers ensures the maximum possible transfection efficiency and cell viability.  No other transfection reagents can match the efficiency, convenience, and gentleness of HT-29 Cell Avalanche™ Transfection Reagent for transfection on HT-29 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

  • Specifically optimized to deliver nucleic acids into HT-29 Cells
  • Highest efficiency to ensure experimental success
  • Extremely gentle to cells
  • 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems
Data:
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on T84 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for T84 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on HT-29 cells by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as HT-29 Cell Avalanche Transfection Reagent.
 
For Other Cells:
HT-29 Cell Avalanche™ Transfection Reagent (human colon adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies.

T84 Cell HCT 116 Cell DLD-1 Cell Caco-2 Cell LS 174T Cell LoVo Cell SW480 Cell SW620 Cell 293 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
      Research Use Only. Not for use in diagnostic or therapeutic procedures.

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