Cambio - Excellence in Molecular Biology

Extraction & Purification - RNA

Extraction & Purification - RNA: Clinical

RNA isolation from a range of clinical samples including buccal swabs, blood, tissues etc.

Hybrid-R™ Blood RNA

Hybrid-R™ Blood RNA is a complete kit with ready-to-use reagent for the isolation of total RNA from up to 0.25 ml whole blood sample. This kit utilizes the lysis method of RiboEx™ LS which has a powerful ability of cell-lysis and the purification method based on glassfiber membrane technology. Fast and convenient procedure of Hybrid-R™ Blood RNA takes only 30 minutes for complete preparation of pure RNA....

GeneAll

Catalogue No.DescriptionPack SizePriceQty
GA-315-106GeneAll Hybrid-R Blood RNA 6 prep sample kit (1 per customer) £0.00 Quantity Add to Order
GA-315-150 GeneAll Hybrid-R Blood RNA 50 mini preps £179.60 Quantity Add to Order

Hybrid-R™ Blood RNA

Hybrid-R™ Blood RNA is a complete kit with ready-to-use reagent for the isolation of total RNA from up to 0.25 ml whole blood sample. This kit utilizes the lysis method of RiboEx™ LS which has a powerful ability of cell-lysis and the purification method based on glassfiber membrane technology. Fast and convenient procedure of Hybrid-R™ Blood RNA takes only 30 minutes for complete preparation of pure RNA....

GeneAll

Description

Hybrid-RTM Blood RNA is a complete kit with ready-to-use reagent for the isolation of total RNA from up to 0.25 ml whole blood sample. This kit utilizes the lysis method of RiboExTM LS which has a powerful ability for cell-lysis. The purification method is based on glassfibre membrane technology. Fast and convenient procedure of Hybrid-RTM Blood RNA takes only 30 minutes for complete preparation of pure RNA.

Whole blood sample is homogenized and lysed in RiboExTM LS, a monophasic solution containing phenol and guanidium salt, which rapidly lyse cells and inactivates nucleases. In conventional methods, the erythrocytes of mammalian blood which does not contain nuclei (and therefore, RNA either) should be removed by pre-treatment such as osmotic lysis for the separation of leukocytes from whole blood. This additional treatments increases  the experiment time and the possibility of RNA breakage, followed by decline of RNA-quality.

Hybrid-RTM Blood RNA does not need the additional treatment of blood sample, and whole blood is lysed in RiboExTM LS in just one step. Then addition of chloroform brings about a separation of the lysate into aqueous and organic phases. After phase-separating, DNA and protein remains in the interphase and the organic phase respectively but released RNA exists in the aqueous phase. The aqueous phase is picked and applied to a EzPureTM to eliminate small amount of contaminated DNA and other blood contaminants.

The pass-through is mixed with buffer RB1, RNA binding buffer, and then the mixture is applied to a mini spin column. After a series of washing with buffer RBW and RNW, pure RNA can be eluted by RNase-free water. Hybrid-RTM Blood RNA is suitable for RNA preparation from 0.1 ml to 0.25 ml mammalian whole blood. The typical yield is 3 ug per 0.25 ml whole blood. The purified RNA can be applicable for the isolation of Poly A RNA, Northern blotting, dot blotting, in vitro translation, cloning, RT-PCR, RPA and other analytical procedures.

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Features and Benefits

¦ Preparation time : ~ 30 minutes
¦ Accurate and consistent yield from whole blood
¦ High purity and yield
¦ Sample size : 100 ~ 250 ul / prep
¦ Instant use : No need of additional materials
¦ No ethanol precipitation
¦ No Genomic DNA contamination
¦ Ready for use in northern blotting, dot blotting, in vitro translation, cloning, RT-PCR
   and other analytical procedures

 

Procedure

( as a mastermix)

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Hybrid-R™ Blood RNA

Hybrid-R™ Blood RNA is a complete kit with ready-to-use reagent for the isolation of total RNA from up to 0.25 ml whole blood sample. This kit utilizes the lysis method of RiboEx™ LS which has a powerful ability of cell-lysis and the purification method based on glassfiber membrane technology. Fast and convenient procedure of Hybrid-R™ Blood RNA takes only 30 minutes for complete preparation of pure RNA....

GeneAll

Click here for protocol

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Hybrid-R™ Blood RNA

Hybrid-R™ Blood RNA is a complete kit with ready-to-use reagent for the isolation of total RNA from up to 0.25 ml whole blood sample. This kit utilizes the lysis method of RiboEx™ LS which has a powerful ability of cell-lysis and the purification method based on glassfiber membrane technology. Fast and convenient procedure of Hybrid-R™ Blood RNA takes only 30 minutes for complete preparation of pure RNA....

GeneAll

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If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200