Cambio - Excellence in Molecular Biology

Extraction & Purification - RNA

Extraction & Purification - RNA: Total RNA

Specialised kits for Total RNA purification for various sources.

RiboEx™

RiboEx™is a complete kit with ready-to-use reagents for the isolation of total RNA from samples of human, animal, plant, yeast, bacterial and viral origin. RiboEx™ is based on the disruption of cells in guanidine salt / detergent solution, followed by organic extraction and alcohol precipitation of the RNA, and it allows simultaneous processing of a large number of samples...

GeneAll

Description

Description

RiboExTM is a complete kit with ready-to-use reagents for the isolation of total RNA from samples of human, animal, plant, yeast, bacterial and viral origin. RiboExTM is based on the disruption of cells in guanidine salt / detergent solution, followed by organic extraction and alcohol precipitation of the RNA, and it allows simultaneous processing of a large number of samples. RiboExTM can yield up to 10 ug / 1 mg tissue or up
to 30 ug / 1 x 106 cultured cells of highly purified total RNA. The resulting total RNA is suitable for the isolation of poly A RNA, northern blotting, dot blotting, in vitro translation, cloning, RT-PCR, RNase protection assays and other analytical procedures.

 

Features and Benefits

¦ Format : Monophase solution type
¦ Sample size : Up to 100 mg tissue / 1 ml RiboExTM 
                        Up to 1 x 107 cells / 1 ml RiboExTM
¦ Preparation time : 50 ~ 65 minutes
¦ Typical yield : Up to 10 ug / 1 mg tissue
                        Up to 30 ug/ 1 x 106 cultured cells
¦ High purity : OD260/230 > 2.0, OD260/280 > 1.8
¦ Accurate and consistent yield from animal tissue, cultured cell line, plant, E. coli 
   and various biological samples
¦ Accurate and easy phase separation
¦ Ready for use in RT-PCR, northern blotting, dot blotting, in vitro translation, molecular cloning,
   real-time PCR, RNase protection assays and other analytical procedures

 

Procedure

 

Specification

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Protocols

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References

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Related products

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