Cambio - Excellence in Molecular Biology

Transfection-EZ Biosystems

Transfection-EZ Biosystems: Primary Cells

Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:?

Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines). Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated. 

For cell lines click HERE

To learn why you should use Avalanche® Cell Type-Specific Transfection reagents, click HERE; To learn how EZ Biosystems developed Avalanche® Cell Type-Specific Transection Reagents, click HERE

Human Artery Endothelial Cell Avalanche™ Transfection Reagent

Transfection of primary cells, such as Human Artery Endothelial Cells, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells. Human Artery Endothelial Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on Human Artery Endothelial Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Human Artery Endothelial Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Artery Endothelial Cell Avalanche™ Transfection Reagent for transfection on Human Artery Endothelial Cells

EZ Biosystems

Catalogue No.DescriptionPack SizePriceQty
EZT-HAOE-1-05Human Artery Endothelial Cell Transfection Reagent0.5ml £435.00 Quantity Add to Order
EZT-HAOE-1-15Human Artery Endothelial Cell Transfection Reagent1.5ml £772.00 Quantity Add to Order

Human Artery Endothelial Cell Avalanche™ Transfection Reagent

Transfection of primary cells, such as Human Artery Endothelial Cells, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells. Human Artery Endothelial Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on Human Artery Endothelial Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Human Artery Endothelial Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Artery Endothelial Cell Avalanche™ Transfection Reagent for transfection on Human Artery Endothelial Cells

EZ Biosystems

Cell Information:

Vascular endothelial cells line the entire circulatory system, from the heart to the smallest capillaries. These cells have very distinct and unique functions that are paramount to vascular biology. These cells also play a pivotal role in a diverse range of physiological activities, such as angiogenesis, forming the selective blood barrier, arterial diseases and cancer development.

The Transfection Reagent:

Transfection of primary cells, such as Human Artery Endothelial Cells, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells.

Human Artery Endothelial Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on Human Artery Endothelial Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Human Artery Endothelial Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Artery Endothelial Cell Avalanche™ Transfection Reagent for transfection on Human Artery Endothelial Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

  • Specifically optimized to deliver nucleic acids into Human Artery Endothelial Cells
  • Highest efficiency to ensure experimental success
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
  • Deliver single or multiple plasmids
  • Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
  • Simplest and straightforward protocols
  • Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
  • Compatible with serum
  • Compatible with transfection in any plate formats
  • Developed and manufactured by EZ Biosystems 

The transfection reagent work best for the following Human Artery Endothelial cells:

  • Human Aortic Endothelial Cell,
  • Human Brachiocephalic Artery Endothelial Cell,
  • Human Carotid Artery Endothelial Cell,
  • Human Coronary Artery Endothelial Cell,
  • Human Internal Artery Thoracic Endothelial Cell,
  • Human Pulmonary Artery Endothelial Cell,
  • Human Subclavian Artery Endothelial Cell
Data:
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human aortic endothelial cell after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human aortic endothelial cell. This test result was also confirmed by transfecting other artery endothelial cells mentioned above. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Artery Endothelial Cell Avalanche Transfection Reagent.
 

For Other Cells:
Human Artery Endothelial Cell Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies.

Human Umbilical Vein Endothelial Cell (HUVEC)
Human Umbilical Artery Endothelial Cell (HUAEC)
Human Dermal Microvascular Endothelial Cell (HMVEC)
Human Dermal Lymphatic Microvascular Endothelial Cell (HDLMVEC)
Human Lung Microvascular Endothelial Cell (HLMVEC, HMVEC-L)
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200