Cambio - Excellence in Molecular Biology

Protein Purification

Protein Purification: IMAC Agaroses

IMAC Agaroses

Cambio offers high-performance immobilized metal affinity chromatography (IMAC) resins, based on BioWorks Workbeads in IDA or NTA formats. IDA or NTA Agarose can be loaded with transition metals to obtain different specificities for his-tagged proteins, or for other applications such as purification of phosphorylated proteins. For purification from diluted solutions, IDA or NTA MagBeads are recommended.

IDA Agarose

Cambio offers high-performance immobilized metal affinity chromatography (IMAC) resins, based on BioWorks Workbeads in IDA or NTA formats. IDA or NTA agarose are supplied in unloaded form and can be loaded with transition metals to obtain different specificities for his-tagged proteins, or for other applications such as purification of phosphorylated proteins. If you require unloaded IMAC beads for purification from dilute starting material, then we recommend IDA or NTA MagBeads.

Cambio

Catalogue No.DescriptionPack SizePriceQty
CA-214921IDA Agarose 10ml £74.00 Quantity Add to Order
CA-214935IDA Agarose 50ml £235.00 Quantity Add to Order
CA-214970IDA Agarose 250ml £957.00 Quantity Add to Order
CA-214984IDA Agarose 500 ml £1,694.00 Quantity Add to Order

IDA Agarose

Cambio offers high-performance immobilized metal affinity chromatography (IMAC) resins, based on BioWorks Workbeads in IDA or NTA formats. IDA or NTA agarose are supplied in unloaded form and can be loaded with transition metals to obtain different specificities for his-tagged proteins, or for other applications such as purification of phosphorylated proteins. If you require unloaded IMAC beads for purification from dilute starting material, then we recommend IDA or NTA MagBeads.

Cambio

Unloaded Immobilised Metal Affinity Chromatography (IMAC) Agaroses

Cambio offers high-performance immobilized metal affinity chromatography (IMAC) resins, based on BioWorks Workbeads in IDA or NTA formats. IDA or NTA agarose are supplied in unloaded form and can be loaded with transition metals to obtain different specificities for his-tagged proteins, or for other applications such as purification of phosphorylated proteins. If you require unloaded IMAC beads for purification from dilute starting material, then we recommend IDA or NTA MagBeads

Which IMAC resin should I use to purify my protein (NTA or IDA?)

For immobilized-metal affinity chromatography (IMAC) you can choose between the metal ion being coupled to the resin matrix via either nitrilotriacetic acid (NTA) or iminodiacetic acid (IDA). There are other chelating ligands used for IMAC resins, but NTA and IDA are the popular ones. Which chelating ligand should you use and why? Does it make a difference?

NTA

  • Tetravalent, coordination number 4
  • Higher binding specificity
  • Stronger coordination of metal ions
  • Lower metal ion leaching


IDA

  • Trivalent, coordination number 3
  • Generally, more non-specific binding
  • Increased metal ion leaching
  • Higher metal loading density
  • Requires lower eluent imidazole concentration
  • Generally, lower yield

 

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