Cambio - Excellence in Molecular Biology

PCR

PCR : Lyophilised PCR reagents

Cirrus™ reagents are Fluorogenics’s next generation of ambient stored quantitative PCR/RT- PCR mastermixes manufactured using their proprietary reagent stabilisation process and have enhanced PCR sensitivity and linearity in hydrolysis and conformational probe amplification. Cirrus™ RNA mastermix provides a simple to use one-tube RT-PCR reaction incorporating a thermostable RNA-dependent DNA polymerase for enhanced cDNA generation at elevated temperatures. Shipped and stored at room temperature, Cirrus™ reagents are ideal for minimizing your lab’s carbon footprint and also very well suited to fieldwork where shipping is an issue.

Cirrus™ RNA Master

CCirrus™ RNA is Fluorogenics’s next generation ambient stored quantitative RT-PCR mastermix manufactured using their proprietary reagent stabilisation process and has enhanced PCR sensitivity and linearity in hydrolysis and conformational probe amplification. Cirrus™ RNA provides a simple to use one- tube RT-PCR reaction incorporating a thermostable RNA-dependent DNA polymerase for enhanced cDNA generation at elevated temperatures. Fluorogenics’s mastermixes are stable for 2 years at room temperature (15-30 C) and shipping is performed ice/dry-ice-free. Benefits of Fluorogenics’s lyophilised qPCR and qRT-PCR mastermixes: • Less pipetting: avoid volumetric errors and reduce contamination in your PCR reactions • Take advantage of Hot-startTM PCR – you don’t need a special PCR programme – this just gets your reaction started in the thermocycler as opposed to on the bench! • Shipping and storage at room temperature • Minimise your carbon footprint

Fluorogenics

Catalogue No.DescriptionPack SizePriceQty
CIR-RNA-1000-01Cirrus™ RNA Master 100 x 25 µL reactions (2 vials) £70.00£70.00Offer until : 31-Aug-2019Offer Code : SUM19Starts 1st July 2019 View Offer Quantity Add to Order
CIR-RNA-1000-05Cirrus™ RNA Master 5 pack of CIR-RNA-1000-01 £332.50£332.50Offer until : 31-Aug-2019Offer Code : SUM19Starts 1st July 2019 View Offer Quantity Add to Order
CIR-RNA-1000-10Cirrus™ RNA Master 10 pack of CIR-RNA-1000-01 £630.00£630.00Offer until : 31-Aug-2019Offer Code : SUM19Starts 1st July 2019 View Offer Quantity Add to Order
CIR-RNA-1000-15Cirrus™ RNA Master 15 pack of CIR-RNA-1000-01 £919.00£919.00Offer until : 31-Aug-2019Offer Code : SUM19Starts 1st July 2019 View Offer Quantity Add to Order

Cirrus™ RNA Master

CCirrus™ RNA is Fluorogenics’s next generation ambient stored quantitative RT-PCR mastermix manufactured using their proprietary reagent stabilisation process and has enhanced PCR sensitivity and linearity in hydrolysis and conformational probe amplification. Cirrus™ RNA provides a simple to use one- tube RT-PCR reaction incorporating a thermostable RNA-dependent DNA polymerase for enhanced cDNA generation at elevated temperatures. Fluorogenics’s mastermixes are stable for 2 years at room temperature (15-30 C) and shipping is performed ice/dry-ice-free. Benefits of Fluorogenics’s lyophilised qPCR and qRT-PCR mastermixes: • Less pipetting: avoid volumetric errors and reduce contamination in your PCR reactions • Take advantage of Hot-startTM PCR – you don’t need a special PCR programme – this just gets your reaction started in the thermocycler as opposed to on the bench! • Shipping and storage at room temperature • Minimise your carbon footprint

Fluorogenics

Cirrus™ RNA is a core mastermix that has been formulated by Fluorogenics specifically as a 5’ nuclease real-time PCR reagent. It contains the core ingredients to amplify 

Cirrus™ RNA

RNA targets requiring only the addition of your primers, dual labelled probes and RNA template.

The core components include a leading brand Taq polymerase and an anti-Taq antibody blended with a thermostable RNase H ve RNA-dependent DNA polymerase (MMuLV). This has the advantage that your PCR reaction should only start once the anti-Taq antibody becomes denatured in your thermocycler. A single tube reverse transcription reaction may be carried out at high temperatures (40-55°C) for specific RNA target PCR amplification.

This dry reagent surpasses the performance of non-lyophilised reverse transcriptase products at a competitive price and flexible format. Each vial can be reconstituted for 50 x 25 ul reactions.

Cirrus™ products are shipped at ambient temperature, without excessive packaging, or the requirement for dangerous goods transport (dry ice). The product has a significantly lower carbon footprint than competing products.

 

 

 

 

 

 

 

 

 

 

 

 

 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Cirrus™ RNA Master

CCirrus™ RNA is Fluorogenics’s next generation ambient stored quantitative RT-PCR mastermix manufactured using their proprietary reagent stabilisation process and has enhanced PCR sensitivity and linearity in hydrolysis and conformational probe amplification. Cirrus™ RNA provides a simple to use one- tube RT-PCR reaction incorporating a thermostable RNA-dependent DNA polymerase for enhanced cDNA generation at elevated temperatures. Fluorogenics’s mastermixes are stable for 2 years at room temperature (15-30 C) and shipping is performed ice/dry-ice-free. Benefits of Fluorogenics’s lyophilised qPCR and qRT-PCR mastermixes: • Less pipetting: avoid volumetric errors and reduce contamination in your PCR reactions • Take advantage of Hot-startTM PCR – you don’t need a special PCR programme – this just gets your reaction started in the thermocycler as opposed to on the bench! • Shipping and storage at room temperature • Minimise your carbon footprint

Fluorogenics

Protocol

Datasheet

 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200