Cambio - Excellence in Molecular Biology

Enzymes for Molecular Biology

Enzymes for Molecular Biology: Enzymes for Molecular Biology

Alkaline Phosphatase (Calf intestine)

Purified from calf intestinal mucosa, this enzyme catalyses the hydrolysis of phosphate monoesters

Cambio

Catalogue No.DescriptionPack SizePriceQty
PM-1025-01Alkaline Phosphatase1,000U £52.00 Quantity Add to Order
PM-1025-02Alkaline Phosphatase5,000U £190.00 Quantity Add to Order

Alkaline Phosphatase (Calf intestine)

Purified from calf intestinal mucosa, this enzyme catalyses the hydrolysis of phosphate monoesters

Cambio

Purified from calf intestinal mucosa, this enzyme catalyses the hydrolysis of phosphate monoesters. Calf intestine alkaline phosphatase, rather than bacterial enzyme is often chosen due to its high turnover rate and milder conditions of inactivation. AP can be used to remove 5'-phosphates from DNA or RNA prior to 5'-end labelling. AP is also used to remove 5'- phosphates from linearised vector molecules to prevent self-ligation of the vector during cloning procedures. The enzyme is tested for endonuclease and ribonuclease contamination.

Unit Definition:

One unit is the amount of enzyme required to hydrolyse 1nm of p-nitrophenylphosphate in 1 min at 37°C in a buffer of 1.0M diethanolamine, 10mM p-nitrophenylphosphate, 0.25mM MgCl2 , pH 9.8.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Alkaline Phosphatase (Calf intestine)

Purified from calf intestinal mucosa, this enzyme catalyses the hydrolysis of phosphate monoesters

Cambio

Technical datasheet

 

Alkaline_Phosphatase.pdf

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200