The location of X- and Y-linked amelogenin sequences can be determined by means of the polymerase chain reaction (PCR) and two primers (AMELF and AMELR). The forward and reverse primers (identical in sequence of the X and Y) span the first intron, but produce different sized X (542bp) and Y (358bp) fragments because of the differences in the sizes of the introns on X and Y. The Cambio kit contains these two primers and a control DNA of known sex.
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