Cambio - Excellence in Molecular Biology

Extraction & Purification - DNA

Extraction & Purification - DNA: Microbial

Specialised DNA purification kits for the isolation of a variety of microbial samples, including viruses, gram positive and gram negative bacteria and fungal materials.

MasterPure™ Gram Positive DNA Purification Kit

The MasterPure™ Gram Positive DNA Purification Kit provides all of the reagents needed to purify DNA from gram positive bacteria

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
MGP04100MasterPure™ Gram Positive DNA Purification Kit100 reactions £228.00£228.00Offer until : 31-Aug-2019Offer Code : SUM19Starts 1st July 2019 View Offer Quantity Add to Order

MasterPure™ Gram Positive DNA Purification Kit

The MasterPure™ Gram Positive DNA Purification Kit provides all of the reagents needed to purify DNA from gram positive bacteria

BioSearch Tech (Lucigen/Epicentre)

The MasterPure™ Gram-Positive DNA Purification Kit provides all of the reagents needed to purify DNA from Gram-positive bacteria. These bacteria lyse more readily after treatment with Ready-Lyse™ Lysozyme and the Gram-Positive Cell Lysis Solution included in the kit. Ready-Lyse Lysozyme is a stable solution of a recombinant lysozyme from a nonmammalian, nonavian source. It has high specific activity and does not bind DNA.1,2

Examples of Gram-positive bacteria tested with this kit are listed in Table 1. The size range of DNA purified by the MasterPure Gram-Positive DNA Purification Kit is shown in Fig. 1. The DNA purified using the kit is suitable for PCR analysis (Fig. 2), and other molecular biology applications

Incubation Times Needed for DNA Recovery from 1ml of Gram Positive Bacterial Culture

Species

Culture Medium

Ready-Lyse™ Incubation

DNA Yield µg/ml

Bacillus subtilis

Brain-Heart Infusion (BHI)

30 min

9.0

Listeria monocytogenes

BHI

Overnight

3.3

Staphylococcus aureus

BHI

Not needed

8.0

Streptococcus mutans

Todd-Hewitt

Overnight

3.0

Lactococcus lactis

M17

30 min

1.1

 

Figure 1Figure 1. Electrophoretic Analysis of DNA Purified Using the MasterPure™ Gram Positive DNA Purification Kit. A. DNA from Bacillus subtilis (ATCC 6051) was separated on a 1% agarose gel and stained with SYBR® Gold.Lane 1, kilobase ladder. Lane 2, 300 ng of B. subtilis DNA.B. Pulsed field gel electrophoresis of Bacillus subtilis (ATCC 6051) DNA. Lane 1, Phage lambda ladder. Lane 2, 300 ng of DNA. Lane 3, 600ng of DNA.


B. subtilis DNA
black right arrow Figure 2Figure 2. PCR Product from DNA Purified Using the MasterPure™ Gram Positive DNA Purification Kit. B. subtilis  DNA (1ul containing 0.5 ng) was amplified by PCR using forward primer 5'-AGG GTC ATT GGA AAC TGG G and reverse primer 5'-CGT GTT GTA GCC CAG GTC ATA. The cycle conditions were 95°C for 2 min and then 30 cycles of [95°C for 45 sec; 55°C for 45 sec; 72°C for 45 sec] followed by 72°C for 2 min. Lane 1, 100 bp ladder. Lane 2, PCR product from 0.5 ng of B. subtilis DNA. Lane 3, no template PCR

PCR of BacF/BacR

Figure 1. Electrophoretic Analysis of DNA Purified Using the MasterPure™ Gram Positive DNA Purification Kit. A. DNA from Bacillus subtilis (ATCC 6051) was separated on a 1% agarose gel and stained with SYBR® Gold.Lane 1, kilobase ladder. Lane 2, 300 ng of B. subtilis DNA. B. Pulsed field gel electrophoresis of Bacillus subtilis (ATCC 6051) DNA. Lane 1, Phage lambda ladder. Lane 2, 300 ng of DNA. Lane 3, 600ng of DNA.

Figure 2. PCR Product from DNA Purified Using the MasterPure™ Gram Positive DNA Purification Kit. B. subtilis DNA (1ul containing 0.5 ng) was amplified by PCR using forward primer 5'-AGG GTC ATT GGA AAC TGG G and reverse primer 5'-CGT GTT GTA GCC CAG GTC ATA. The cycle conditions were 95°C for 2 min and then 30 cycles of [95°C for 45 sec; 55°C for 45 sec; 72°C for 45 sec] followed by 72°C for 2 min. Lane 1, 100 bp ladder. Lane 2, PCR product from 0.5 ng of B. subtilis DNA. Lane 3, no template PCR.

 




If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

MasterPure™ Gram Positive DNA Purification Kit

The MasterPure™ Gram Positive DNA Purification Kit provides all of the reagents needed to purify DNA from gram positive bacteria

BioSearch Tech (Lucigen/Epicentre)

Protocols for: MasterPure™ Gram Positive DNA Purification Kit

MasterPure™ Gram Positive DNA Purification Kit Protocol

(catalogue number MGP04020 / MGP04100)

Please note: all protocols off site are the responsibility of the products supplier

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

MasterPure™ Gram Positive DNA Purification Kit

The MasterPure™ Gram Positive DNA Purification Kit provides all of the reagents needed to purify DNA from gram positive bacteria

BioSearch Tech (Lucigen/Epicentre)

References

  1. Hoffman, L. and Jarvis, B. (2003) EPICENTRE Forum 10(3), 3.
  2. Jarvis, B. and Hoffman, L. (2004) EPICENTRE Forum 11(3), 7.
  3. Luna, V.A. et al. (2006) J. Clin. Microbiol. 44, 2367.
  4. Li, J. et al. (2007) Immunol. 75, 1811.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

MasterPure™ Gram Positive DNA Purification Kit

The MasterPure™ Gram Positive DNA Purification Kit provides all of the reagents needed to purify DNA from gram positive bacteria

BioSearch Tech (Lucigen/Epicentre)

Epicentre® Forum Get your Epicentre® Forum - hot off the press!
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If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

MasterPure™ Gram Positive DNA Purification Kit

The MasterPure™ Gram Positive DNA Purification Kit provides all of the reagents needed to purify DNA from gram positive bacteria

BioSearch Tech (Lucigen/Epicentre)

Applications

  • Purification of genomic DNA from Gram-positive bacteria for fosmid library construction, PCR, restriction digests, and Southern blotting.

Benefits

  • PCR-ready DNA.
  • Scaleable method for large sample volumes.
  • High-molecular-weight DNA
  • Lysozyme included; no separate purchase required.
  • Tested on a variety of species.
  • Gram-negative bacteria are also lysed.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200