Cambio - Excellence in Molecular Biology

CRISPR-Cas 9

CRISPR-Cas 9: Reagents for CRISPR-Cas9

Reagents and kits used in CRISPR/Cas 9

T7-Scribe™, and SP6-Scribe™ Standard RNA IVT kits

T7-Scribe and SP6-Scribe Standard RNA IVT kits are specially formulated to utilise high concentrations of NTPs that are inhibitory to other in vitro transcription systems. For the production of in vitro transcribed RNA with T7/SP6 RNA polymerase (depending on kit) and canonical (GAUC) NTPs. A reaction produces 150 µg RNA from 1 µg DNA in 2 hours.

CellScript

Catalogue No.DescriptionPack SizePriceQty
C-AS3106SP6-Scribe™ Standard RNA IVT kit50 Reactions £193.00 Quantity Add to Order
C-AS3107T7-Scribe™ Standard RNA IVT kit50 Reactions £193.00 Quantity Add to Order

T7-Scribe™, and SP6-Scribe™ Standard RNA IVT kits

T7-Scribe and SP6-Scribe Standard RNA IVT kits are specially formulated to utilise high concentrations of NTPs that are inhibitory to other in vitro transcription systems. For the production of in vitro transcribed RNA with T7/SP6 RNA polymerase (depending on kit) and canonical (GAUC) NTPs. A reaction produces 150 µg RNA from 1 µg DNA in 2 hours.

CellScript

The SP6-Scribe™ Standard RNA IVT Kit is specially formulated to enable users to obtain the maximum possible yields of canonical (GAUC) RNA from an in vitro transcription (IVT) reaction. The standard 2 hour, 20 μl reaction will yield up to 90 μg of RNA from 1 μg of the control template. These yields are made possible by the high-performance properties of the SP6-Scribe enzyme.

 The SP6-Scribe Standard RNA IVT Kit produces exceptionally high yields of either long or short transcripts. The standard reaction can be scaled up to produce milligram amounts of RNA. In addition, an SP6-Scribe reaction can be readily modified to prepare fluorescent-, biotinylated- or digoxigenin-labeled RNA.

SP6-Scribe IVT RNA can be processed into mRNA (5'-end capped and 3'-end poly(A) tailed) through the use of CELLSCRIPT's ScriptCap™ m7G Capping System, ScriptCap 2'-O-Methyltransferase Kit and A-Plus™ Poly(A) Polymerase Tailing Kit (available separately).

The T7-Scribe™ Standard RNA IVT Kit is specially formulated to enable users to obtain the maximum possible yields of canonical (GAUC) RNA from an in vitro transcription (IVT) reaction. The standard 2 hour, 20 μl reaction will yield up to 150 μg of RNA from 1 μg of the control template. These yields are made possible by the high-performance properties of the T7-Scribe enzyme.

The T7-Scribe Standard RNA IVT Kit produces exceptionally high yields of either long or short transcripts. The standard reaction can be scaled up to produce milligram amounts of RNA. In addition, a T7 -Scribe reaction can be readily modified to prepare fluorescent-, biotinylated- or digoxigenin-labeled RNA.

T7-Scribe IVT RNA can be processed into mRNA (5'-end capped and 3'-end poly(A) tailed) through the use of CELLSCRIPT's ScriptCap™ m7G Capping System, ScriptCap 2'-O-Methyltransferase Kit and A-Plus™ Poly(A) Polymerase Tailing Kit (available separately).

  

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

T7-Scribe™, and SP6-Scribe™ Standard RNA IVT kits

T7-Scribe and SP6-Scribe Standard RNA IVT kits are specially formulated to utilise high concentrations of NTPs that are inhibitory to other in vitro transcription systems. For the production of in vitro transcribed RNA with T7/SP6 RNA polymerase (depending on kit) and canonical (GAUC) NTPs. A reaction produces 150 µg RNA from 1 µg DNA in 2 hours.

CellScript

Protocols for: SP6-Scribe™ and T7-Scribe™ Standard RNA IVT kits

Due to the constant updating of the protocols by the manufacturer we have provided a direct link to CELLSCRIPT'’s product page, where the latest protocol is available.
Please note this will open a new page or window on your computer.
 
 
Please note: all protocols are the responsibility of the product supplier

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

T7-Scribe™, and SP6-Scribe™ Standard RNA IVT kits

T7-Scribe and SP6-Scribe Standard RNA IVT kits are specially formulated to utilise high concentrations of NTPs that are inhibitory to other in vitro transcription systems. For the production of in vitro transcribed RNA with T7/SP6 RNA polymerase (depending on kit) and canonical (GAUC) NTPs. A reaction produces 150 µg RNA from 1 µg DNA in 2 hours.

CellScript

1.Karikó, K. et al., (2008) Molecular Therapy 16, 1833.

2.Anderson, B.R. et al., (2010) Nucl. Acids Res. 17, 5884.

3.Karikó, K. et al., (2005) Immunity 23, 165.

4.Karikó, K. and Weissman, D. (2007) Curr. Opin. Drug Discov. Devel. 10, 523.

5.Sambrook, J. et al., (1989) Molecular Cloning: A Laboratory Manual (2nd ed.), New York, Cold Spring Harbor Laboratory Press

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

T7-Scribe™, and SP6-Scribe™ Standard RNA IVT kits

T7-Scribe and SP6-Scribe Standard RNA IVT kits are specially formulated to utilise high concentrations of NTPs that are inhibitory to other in vitro transcription systems. For the production of in vitro transcribed RNA with T7/SP6 RNA polymerase (depending on kit) and canonical (GAUC) NTPs. A reaction produces 150 µg RNA from 1 µg DNA in 2 hours.

CellScript

  • Use AmpliCap™ High Yield Message Maker Kits for efficient production of RNA with an m7G[5´]ppp[5´]G cap structure on the 5´ end.
  • Because of the very high concentration of NTPs used in AmpliScribe reactions, these kits are not recommended for preparing high specific-activity radiolabeled RNA probes.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

T7-Scribe™, and SP6-Scribe™ Standard RNA IVT kits

T7-Scribe and SP6-Scribe Standard RNA IVT kits are specially formulated to utilise high concentrations of NTPs that are inhibitory to other in vitro transcription systems. For the production of in vitro transcribed RNA with T7/SP6 RNA polymerase (depending on kit) and canonical (GAUC) NTPs. A reaction produces 150 µg RNA from 1 µg DNA in 2 hours.

CellScript

Applications

  • High-yield synthesis of RNA from DNA cloned downstream from a T7, T3, or SP6 RNA polymerase promoter, for microinjection, RNA interference, or antisense experiments.
  • Structural and functional studies of splicing, processing, heterogeneous nuclear RNAs, tRNAs, viral RNAs, and ribozymes.1
  • Production of fluorescent2-, biotinylated3-, or digoxigenin4- labeled RNA



Benefits

  • High yields of RNA from all templates—synthesize both long (Fig. 2) and short RNA using only one kit.5 Produce microgram amounts of RNA from as little as 1 ng of template.
  • Reactions can be scaled up to produce milligram amounts of RNA.
  • Produce >20 times more RNA than conventional or "homemade" in vitro transcription reactions.
  • RNase inhibitor included.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200