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Lab Reagents

Lab Reagents: Absolute Lectins

Absolute Lectins, perfect for Glycoscience Medicago is a primary manufacturer of a wide variety of exceptionally pure lectins purified by affinity chromatography. Lectins are non-enzymatic proteins of non-immunoglobulin origin that bind specifically and reversibly to carbohydrate moieties without altering the covalent structure of the glycosyl ligands. The term ‘lectin’ is derived from the latin word legere, meaning ‘to select’. The specificity of a lectin is usually defined by the monosaccharides or oligosaccharides that are best at inhibiting the agglutination or precipitation caused by the lectin. Lectins are usually of plant origin but do occur in many types of organism; some are glycoproteins and may be soluble or membrane-bound. The biological function of lectins is far from fully explored. Their specificity enables binding to glycoproteins and polysaccharides as well as agglutination of erythrocytes and stimulation of blood lymphocytes. Because of their ability to distinguish glycosyl ligands on human red blood cells, lectins can be used for blood typing.Immobilized lectins can be used in affinity chromatography to purify and isolate glycoproteins, glycolipids, polysaccharides, viruses and cells. Carbohydrate-containing substances bound to the lectin may be eluted with a competitive binding substance. Medicago offers lectins as a freeze-dried powder or as a solution manufactured under aseptic conditions. If you don’t find the lectin you are looking for, we can certainly produce it for you. Furthermore, we offer different custom lectin conjugates on request. •Ultrapure quality •High activity •Wide range •Lyophilized powder •Custom lectins and conjugates

Narcissus pseudonarcissus lectin (NPA/NPL)

Narcissus pseudonarcissus lectin (NPL) is isolated from daffodils. It exists as a dimeric protein with the molecular weight of 26 kDa composed by two subunits of 13 kDa each. The lectin has specificity toward alpha linked mannose. NPL is used in characterisation of early stages of apoptosis and is mitogenic for human lymphocytes The lectin agglutinates rabbit erythrocytes but is non-reactive with human erythrocytes

Medicago AB

Catalogue No.DescriptionPack SizePriceQty
MC-05-0119-10Narcissus pseudonarcissus lectin (NPA/NPL) 10 mg £161.00 Quantity Add to Order
MC-05-0119-50Narcissus pseudonarcissus lectin (NPA/NPL) 50 mg £575.00 Quantity Add to Order

Narcissus pseudonarcissus lectin (NPA/NPL)

Narcissus pseudonarcissus lectin (NPL) is isolated from daffodils. It exists as a dimeric protein with the molecular weight of 26 kDa composed by two subunits of 13 kDa each. The lectin has specificity toward alpha linked mannose. NPL is used in characterisation of early stages of apoptosis and is mitogenic for human lymphocytes The lectin agglutinates rabbit erythrocytes but is non-reactive with human erythrocytes

Medicago AB

Product description

Narcissus pseudonarcissus lectin (NPL) is isolated from daffodils. It exists as a dimeric protein with the molecular weight of 26 kDa composed by two subunits of 13 kDa each. The lectin has specificity toward alpha linked mannose. NPL is used in characterisation of early stages of apoptosis and is mitogenic for human lymphocytes (1). The lectin agglutinates rabbit erythrocytes but is non-reactive with human erythrocytes (2).

Medicago’s NPL lectin is supplied as a white lyophilized powder from a buffer containing 10 mM CH3COONH4. The purity is determined by SDS-PAGE, which generates one band at 13 kDa, corresponding to the two isomers (the lectin dissociates below pH 5.0 and above pH 9.0). The lectin is available in vials containing 50 mg or 10 mg lyophilized powder and the product is to be used for laboratory work only.

Directions for use

The lectin may be reconstituted with 2 ml of deionized water before use. Spin the vial gently until full dissolution. Aggregation is thought to occur in the presence of high concentrations of 2-mercaptoethanol. The solution may be reconstituted in this buffer to desired working concentration.

 

Shipping and storage

The product is shipped at -20°C however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than five years from production date when stored below -20°C. After reconstitution with deionized water, the solution may be stored frozen in working aliquots for up to 12 month

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Narcissus pseudonarcissus lectin (NPA/NPL)

Narcissus pseudonarcissus lectin (NPL) is isolated from daffodils. It exists as a dimeric protein with the molecular weight of 26 kDa composed by two subunits of 13 kDa each. The lectin has specificity toward alpha linked mannose. NPL is used in characterisation of early stages of apoptosis and is mitogenic for human lymphocytes The lectin agglutinates rabbit erythrocytes but is non-reactive with human erythrocytes

Medicago AB

References

(1) Summers C., Forrest J., Norval M., Sharp J. M. (2002) The potenitally insecticidal Narcissus pseudonarcissus lectin demonstrates age-related mitogenecity. FEMS immunology and medical microbiology vol 33 Issue 1, 47–9.

(2) Van Damme J. M., Allen A. K., Peumans W. J. (2007). Related mannose-specific lectins from different species of the family Amaryllidaceae. Physiologia Plantarum Vol 53, Issue 1, 52–7.

(3) Structure of Narcissus pseudonarcissus lectin complex with Mannobiose at 1.7 A resolution, form II Rizkallah, P.J., Ozbey, S.,
Sauerborn, M.K. To be Published. Where did we got the picture?

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Narcissus pseudonarcissus lectin (NPA/NPL)

Narcissus pseudonarcissus lectin (NPL) is isolated from daffodils. It exists as a dimeric protein with the molecular weight of 26 kDa composed by two subunits of 13 kDa each. The lectin has specificity toward alpha linked mannose. NPL is used in characterisation of early stages of apoptosis and is mitogenic for human lymphocytes The lectin agglutinates rabbit erythrocytes but is non-reactive with human erythrocytes

Medicago AB

Specifications

Appearance: White lyophilized powder or flocculate                              

Source: Daffodils

Molecular weight: 26 kDa 

Sugar specificity: D-GalNAc

Activity: Agglutinates rabbit erythrocytes at <0.1 μg/ml, inhibited by 10 mM D-galactose. NPL agglutinates both normal and trypsine treated rabbit erythrocytes.

Microorganisms: < 100 CFU/g

Protein content: > 90 %, OD280nm, Abs 280 nm (ε 1.35 = 1 mg/mL)

Identity: SDS-PAGE, one band at 13 kDa

Shelf life: > Five years when stored at -20°

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Narcissus pseudonarcissus lectin (NPA/NPL)

Narcissus pseudonarcissus lectin (NPL) is isolated from daffodils. It exists as a dimeric protein with the molecular weight of 26 kDa composed by two subunits of 13 kDa each. The lectin has specificity toward alpha linked mannose. NPL is used in characterisation of early stages of apoptosis and is mitogenic for human lymphocytes The lectin agglutinates rabbit erythrocytes but is non-reactive with human erythrocytes

Medicago AB

Applications

  • Mitogenic studies of human lymphocytes
  • Agglutination studies

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200