Cambio - Excellence in Molecular Biology

PCR & qPCR

PCR & qPCR: Reagents

Accessories for your PCR experiment

PARR™ Buffer

The PARR™ Buffer has been designed by Professor Martin Evans and his colleagues in Cambridge exclusively for Cambio Limited to optimise and improve the yield of DNA amplified by PCR

Cambio

Catalogue No.DescriptionPack SizePriceQty
CA-1149-01PARR™ buffer with magnesium1ml £5.00£5.00Offer until : 31-Aug-2019Offer Code : SUM19Starts 1st July 2019 View Offer Quantity Add to Order
CA-1149-02PARR™ buffer with magnesium10ml £40.00£40.00Offer until : 31-Aug-2019Offer Code : SUM19Starts 1st July 2019 View Offer Quantity Add to Order
CA-1149-10PARR™ buffer with magnesium100ml £190.00£190.00Offer until : 31-Aug-2019Offer Code : SUM19Starts 1st July 2019 View Offer Quantity Add to Order
CA-1149-20PARR™ buffer with magnesium>100ml £375.00£375.00Offer until : 31-Aug-2019Offer Code : SUM19Starts 1st July 2019 View Offer Quantity Add to Order

PARR™ Buffer

The PARR™ Buffer has been designed by Professor Martin Evans and his colleagues in Cambridge exclusively for Cambio Limited to optimise and improve the yield of DNA amplified by PCR

Cambio

This buffer has been designed by Professor Martin Evans and his colleagues in Cambridge exclusively for Cambio Limited to optimise and improve the yield of DNA amplified by PCR. Typically customers have reported two-fold increases in yields of PCR products.

This buffer contains amongst other components, special reagents which enhance the binding of the polymerase to its substrates. It is ready-made, specifically to avoid any possible contamination which could confuse the results of PCR amplification. Customers are assured of a reliable, regular and reproducible source of the buffer, which is available with or without magnesium.

Technical Information

This PCR optimised buffer is supplied at x10 concentration. The pH (at 25°C) is nominally 8.00 with a final magnesium concentration of 1.5mM. A final nucleotide concentration of 200µM should be used together with up to 0.5µg of each primer. The magnesium-free version of PARR buffer allows titrations to be performed with magnesium ions whilst at the same time adjusting the nucleotide concentration. 

Note: magnesium should be added directly to the reaction mixture at the required concentration and not to the stock PARR buffer.



If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

PARR™ Buffer

The PARR™ Buffer has been designed by Professor Martin Evans and his colleagues in Cambridge exclusively for Cambio Limited to optimise and improve the yield of DNA amplified by PCR

Cambio

Download protocol here

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

PARR™ Buffer

The PARR™ Buffer has been designed by Professor Martin Evans and his colleagues in Cambridge exclusively for Cambio Limited to optimise and improve the yield of DNA amplified by PCR

Cambio

Note: magnesium should be added directly to the reaction mixture at the required concentration and not to the stock PARR buffer.

Sample Policy

A £5 fee to cover administration costs will be charged for sample packs. If feedback is received within 6 months of purchase, this fee, will be credited back to you.

 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200