Cambio - Excellence in Molecular Biology

Transfection-EZ Biosystems

Transfection-EZ Biosystems: Cell Lines

Avalanche®- Cell Line-specific Transfection Reagent series:

Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines). Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.

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Learn why you should use Avalanche® Cell Type-Specific Transfection reagents

 

How EZ Biosystems developed Avalanche® Cell Type-Specific Transection Reagents

 



Micro-Proteofection Kit VI

Cell culture, proteofection and high-resolution microscopy directly in the culture vessel

Biontex

Catalogue No.DescriptionPack SizePriceQty
K030-0.25 Micro-Proteofection Kit VI250ul £1,037.00 Quantity Add to Order

Description

Like the μ-Transfection Kit VI (cat. no. K010), μ-Proteofection Kit VI, developed especially for proteofection, is a unique combination of μ-Slides VI from ibidi and PROTEOfectene™ from Biontex. The high-performance kit enables living proteofected cells to be observed under high-resolution microscopy.

In the ultra-simple protocol, adherent cells are treated in the μ-Slide with proteoplexes formed from PROTEOfectene™ and a corresponding protein, and can subsequently be observed directly in excellent optical quality. Examples of applications include tracking the progress of a fluorescence-tagged protein through the cell from endocytosis to its place of action.

  • Immediate live observation possible
  • Ideal optical properties for transmitted light and fluorescence microscopy
  • Optimum cell vitality
  • Full serum compatibility
  • Visualization of transfection process in living cells
  • Efficient, rapid protocol
  • Cells can be fixed and dyed
  • Compatible with oil immersion

 

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References

E. Conti, J. Kuriyan; Structure Fold. Des. v8, 329 – 338 (2000) [PubMed ID: 10745017]

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Notes

  
   
   
   
   
   
   

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Applications & Benefits

Application Proteofection and high-resolution microscopy of living eukaryotic cells (live cell imaging)
Content μ-Slides VI (6 channels each), PROTEOfectene™, R-Phycoerythrin as positive control
Assays 90 per kit
Shipping At room temperature
Storage 4°C
Shelf Life 1 year (after date of delivery)

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