Cambio - Excellence in Molecular Biology

Oligo Synthesis

Oligo Synthesis : Purification

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

Seal for unused wells on Adapter Rack

Seal for unused wells on Adapter Rack

Glen Research

Catalogue No.DescriptionPack SizePriceQty
60-0020-01Seal for unused wells on Adapter Rack Pk/1 £60.00£55.20Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

Seal for unused wells on Adapter Rack

Seal for unused wells on Adapter Rack

Glen Research

Seal for unused wells on Adapter Rack

Catalog Number: 60-0020-xx

Description: Seal for unused wells on Adapter Rack

POLY-PAK™ PURIFICATION

The use of Poly-Pak™ packings in cartridges or barrels overcomes several disadvantages usually associated with reverse phase (RP) cartridges. The packing is stable in the pH range 1-13, thus the ammonium hydroxide solution, diluted with water, is loaded directly onto the packing. Also, after elution of failure sequences, the trityl group is removed and washed from the support-bound oligonucleotide. The fully deprotected product can then be eluted and isolated by lyophilization. Poly-Pak™ Cartridges may also be used for desalting normal or labelled oligonucleotides. The original Poly-Pak cartridge and barrel are designed for 0.2 µmole syntheses or less. Poly-Pak II cartridges and barrels are designed for use with 1 µmole syntheses. A booklet, User Guide To Poly-Pak™ Cartridge Purification, describes in detail the process and several applications. This booklet is delivered with cartridges, barrels, and packing and is available on request.

 

GLEN-PAK™ PURIFICATION

Glen-Pak? DNA and RNA cartridges have advantages over Poly-Pak cartridges in that a single loading of the diluted crude deprotection solution is all that is necessary. Also, the range of purification has been extended to 100 using DMT-on oligos. Glen-Pak cartridges have similar performance to Fluoro-Pak cartridges but without the need for the fluorous DMT group at the 5' terminus and special phosphoramidites, so the cost is lower. In addition, Glen-Pak cartridges allow purification of virtually the complete range of dyes and modifiers. The Glen-Pak DNA Cartridge 3g is a large cartridge capable of purifying 10-20 µmole oligonucleotide syntheses using the standard DMT-on procedure and Glen-Pak DNA 30mg 96-Well Plates are for parallel purification of up to 50 nmole scale syntheses. Scale suggestions for the Glen-Pak DNA product line are shown below:

Glen-Pak DNA Product

Catalog Number

Synthesis Scale
Compatibility

Glen-Pak DNA 50mg Purification Cartridge

60-5000-96

10 nmole ? 200 nmole

Glen-Pak DNA Purification Cartridge

60-5100-XX
60-5200-XX

10 nmole ? 1.0 µmole

Glen-Pak DNA Cartridge 3G

60-5300-01

5 µmole ? 20 µmole

Glen-Pak DNA 30 mg 96-Well Plate

60-54

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Seal for unused wells on Adapter Rack

Seal for unused wells on Adapter Rack

Glen Research

ILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
60-0020-01 Pk/1 1grams N/A N/A N/A N/A N/A N/A N/A
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm  
Approximate Number of Additions
60-0020-01 Pk/1 1grams N/A N/A N/A N/A N/A  
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm    
Approximate Number of Additions
60-0020-01 Pk/1 1grams N/A N/A N/A N/A

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200