---

Useful Information

Nucleic Acid Information

• Nucleic Acid Data
• 1 pmole of 1,000 bp RNA = 0.33 µg
• 1 pmole of 1,000 bp DNA = 0.66 µg (3.03 pmoles of ends)
• 1 pmole of pUC18/19 DNA (2,686 bp) = 1.77µg
• 1 pmole of lambda DNA (48,502 bp) = 32.01µg

Molecular Weight Conversions

• MW of a double-stranded DNA molecule = (# of base-pairs) x (650 daltons/basepair)
• MW of a single-stranded DNA molecule = (# of bases) x (330 daltons/base)
• MW of a single-stranded RNA molecule = (# of bases) x (340 daltons/base)

Estimating Tm of an Oligonucleotide

T_m = [4°C x # of Cs and Gs in the oligo] + [2°Cx # of As and Ts in the oligo]

Estimating Molar Amounts of Primer

To estimate the weight of 1picomole of oligodeoxynucleotide, multiply the number of nucleotides by 0.33ng.
For example, one picomole of a 24-mer is 7.92ng (24 x 0.33 ng = 7.92ng).

Estimating Molar Amounts of Template

To estimate the weight of 100fmoles of double-stranded DNA template, multiply the number of kilobases by 66ng. For example, 100fmoles of a 2.7kb plasmid is 178ng (2.7 x 66ng = 178ng).

Metric Prefixes
Factor	Prefix	Symbol
109	giga	G
106	mega	M
103	kilo	k
10-3	milli	m
10-6	micro	µ
10-9	nano	n
10-12	pico	p
10-15	femto	f
10-18	atto	a
10-21	zepto	z

Approximate Amount of Template (in nanograms) Required for DNA Sequencing Reactions

Metric Prefixes
Template Required (fmoles)	Template Size (insert + vector) in kilobases
		1 kb	3 kb	5 kb	7 kb	9 kb
	25	17	50	83	120	150
	50	33	100	165	230	300
	75	50	150	250	350	450
	100	66	200	330	460	600
	150	100	300	500	700	900
	200	135	400	660	930	1200
	250	165	500	830	1200	1550
	300	200	600	1000	1400	1800

Protein Yield Information

Bacterial Cells: Escherichia coli or Salmonella typhimurium

Cell data	Per cell	Per litre at 109 cells per ml
Wet Weight	9.5 x 10–13g	0.95g
Dry Weight	2.8 x 10–13g	0.28g
Total Protein	1.55 x 10–13g	0.15g
Volume	1.15µm3=1 femtolitre
Protein conc. in the cell: 135mg/ml

Theoretical maximum yield for a protein of interest from 1 litre of culture (10⁹ cells/ml):

0.1% of total protein: 150µg/litre

2.0% of total protein: 3mg/litre

50.0% of total protein: 75mg/litre

If you have any queries about the products we supply that are not covered here, please get in touch with us by clicking here. or call +44 (0)1954 210 200.