pIndigoBAC-5 (BamH I-Cloning Ready) Vector

Catalogue number

Pack size

BACB085H

500ng

pIndigoBAC-5 (Hind III-Cloning Ready) Vector

Catalogue number

Pack size

BACH095H

500ng

pIndigoBAC-5 Forward Sequencing Primer

Catalogue number

Pack size

BFP0701

1nmole

pIndigoBAC-5 Reverse Sequencing Primer

Catalogue number

Pack size

BRP0801

1nmole

Linearized and dephosphorylated Bacterial Artificial Chromosome (BAC) Vectors

pIndigoBAC-5 is derived from pBeloBAC11 and pIndigoBAC1 and will accommodate and stably maintain DNA inserts of >100kb. pIndigoBAC-5 has been linearized at its unique BamH I or Hind III site, dephosphorylated and highly purified and is ready for cloning your BamH I- or Hind III-cut genomic DNA. The linearized and dephosphorylated vectors are tested to ensure the completeness of linearisation, dephosphorylation and the integrity of the BamH I and Hind III ends. The complete sequence and restriction map of pIndigoBAC-5 is available at www.epibio.com.

Once DNA has been ligated into the pIndigoBAC-5 vector, BAC libraries can be produced using the high efficiency TransforMax™ EC100 Electrocompetent E. coli (available separately). These cells accommodate and stably maintain large BAC clones. Sequencing primers for BAC end-sequencing of pIndigoBAC-5 clones are available separately.

Quality Control:

pIndigoBAC-5 vectors are tested to yield >90% recombinant (white) clones when used to clone BamH I or Hind III DNA fragments.

Figure 1. Cloning-Ready pIndigoBAC-5 Vectors are supplied linearized at either the unique BamH I or Hind III site, completely dephosphorylated, and highly purified.

Last modified: 2008-08-31 11:41:07

Benefits

• Derived from pBeloBAC11 and pIndigoBAC
• Linearised, dephosphorylated and then highly purified and ready for cloning
• Tested to ensure complete linearisation, dephosphorylation and the integrity of the BamH I and Hind III ends
• Enhanced blue/white screening of recombinants

Last modified: 2008-05-14 16:46:46

Protocols for: pIndigoBAC-5 (BamH I & HindIII - Cloning Ready)

pIndigoBAC-5 (BamH I & HindIII - Cloning Ready) Protocol

(catalogue number BACB085H / BACH095H)

pIndigoBAC-5 Sequencing Primers Protocol

(catalogue number BFP0701 / BRP0801)

Please note: all protocols off site are the responsibility of the products supplier

Last modified: 2008-05-14 16:46:46

References

1. Birren, B. et al. (1999) Bacterial Artificial Chromosomes in Genome Analysis: A Laboratory Manual v. 3, 241.
2. Hurowitz, E.H. et al. (2000) DNA Research 7 (2),1.

Last modified: 2008-05-14 16:46:46