mScript™ mRNA Production System
mScript™ Production System
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Last modified: 2008-07-15 14:57:15
The mScript™ mRNA Production System provides a quick and easy way to build a superior capped and polyadenylated mRNA transcript. Based upon a high-yield T7 transcription system, the all-inclusive kit contains the reagents needed to produce high yields of 100% capped mRNA; all you need is the template DNA.
The mScript™ mRNA Production System differs from co-transcriptional mRNA production methods by decoupling the capping and transcription processes. Instead of using a costly and inefficiently incorporated nucleotide cap analog, the mScript™ mRNA Production System uses the Vaccinia virus-derived capping enzyme to add the cap, ensuring 100% capping and 100% proper cap orientation. In addition, the mScript™ system also contains the Vaccinia 2'-O-Methyltransferase enzyme, allowing the natural, translation-boosting Cap 1 structure to be built.
After capping, poly(A) tails of the desired length can be added with the included mScript™ poly(A) polymerase enzyme. Each reaction yields approximately 60ug of fully capped and polyadenylated mRNA ready for use in transfections, microinjections, or in vitro translation systems.
Last modified: 2008-05-14 16:46:46
Benefits
- 100% capping - Virtually all transcripts will be capped; co-transcriptional systems typically cap less than 80%.
- 100% proper cap orientation - Traditional co-transcriptional systems use an expensive cap analog which can be incorporated in the reverse orientation; these transcripts are not translated by the cell, reducing translation efficiency.
- Cap 1 structure - mScript is the only mRNA production system that builds this translation boosting structure; Cap 1 mRNA exhibits up to 50% better translation in vivo.
- Yield - The decoupling of the transcription and capping processes allows for much higher yields than most co-transcriptional system; up to 60ug per reaction.
- Your time - Higher yields, more capping, and the better in vivo translation, all in the same amount of time as the competition.
Last modified: 2008-05-14 16:46:46
