UltraClean™ Fecal DNA Kit

Sample Policy

A £5 fee to cover administration costs will be charged for sample packs. If feedback is received within 6 months of purchase, this fee, will be credited back to you.

Last modified: 2008-05-14 16:46:46

The UltraClean™ Fecal DNA Kit is designed to isolate cellular, PCR quality genomic DNA from stool samples, dried fecal samples, droppings, forensic samples, and gut material. The kit also ensures removal of humic acid inhibitors of PCR from the starting sample.

Fecal material is added directly to a bead beating lysis tube. The addition of a lysis solution, bead solution and Inhibitor Removal Solution allows for efficient cell lysis. The prinicipal is to lyse the microorganisms in the fecal samples by a combination of heat, detergent, and mechanical force against specialized beads. The cellular components are lysed by mechanical action on a vortex using a specially designed Mo Bio vortex adapter. From the lysed cells, the released DNA is bound to a silica spin filter. The filter is washed, and the DNA is recovered in certified DNA-free Tris buffer. This kit has been used by numerous law enforcement agencies, zoologists and forensic scientists.

Equipment required:

Requires MoBio Vortex Adapter for cell lysis ( Cat No. UC-13000-50-V1).

Last modified: 2008-05-14 16:46:46

Benefits

• Rapid Protocol - Purify DNA from 250mg to 1gram of fecal samples in 30 minutes.
• High Quality DNA - Yields PCR ready DNA greater than 50 kb.
• Novel Method - Uses bead beating technology and a proprietary Inhibitor Removal Solution to eliminate humic acid inhibitors of PCR.
• Flexible - Efficiently lyses spores and fungi.
• Easy to Use - Bead tubes and bead solution are separate for easier sample handling.
• Complete - All tubes and reagents required are included in kit and ready to use.

Last modified: 2008-05-14 16:46:46

Protocols for: UltraClean™ Fecal DNA Kit

(catalogue number UC-12811-50 / UC-12811-100)

Please note: all protocols off site are the responsibility of the products supplier

Last modified: 2008-05-14 16:46:46