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The Applications of Transposomics™ Are Limited Only by Our Imagination.
The field of Transposomics™ exploits the ability of certain transposase enzymes to catalyze the random “hop” or insertion of an “artificial” transposon into any other DNA during a brief in vitro or in vivo transposition reaction. The transposon can be any DNA that has a transposase recognition sequence on each end (e.g., Figure 1). An artificial transposon doesn't encode a transposase, but can encode selectable markers, priming sites, origins of replication, RNA polymerase promoters, or other genetic elements or control sequences.
Figure 1. An EZ-Tn5™ Transposon. An EZ-Tn5 Transposon can be any DNA sequence that is between properly-oriented 19-bp inverted repeat Mosaic End (ME) sequences that are specifically and uniquely recognized by EZ-Tn5™ Transposase. The EZ-Tn5 System is based on the hyperactive Tn5 in vitro transposition system described by IY Goryshin & WS Reznikoff1. This system retains the insertion characteristics of Tn5–the most random transposon system known–but has a transposition frequency 1000-fold higher than wild-type Tn5. EPICENTRE also has a HyperMu™ In Vitro Transposition System that is 50-100 times more active than other MuA Transposase Systems.
- Applications of In Vitro Transposomics™
- Transposon Nomenclature
- Criteria for Choosing a Transposon System