X-Y FISH Bovine Sex Test Kit - X Biotin; Y Cy3 labelled

Catalogue number	Pack size	Price
CA-1611	10 tests	£1236 (GBP)

X and Y chromosome paints were developed from sorted yak chromosomes for sexing cattle spermatozoa.

The procedure was evaluated using the Beltsville sperm sexing technology, which separates spermatozoa by flow-cytometry into X- and Y-bearing fractions. Using FISH, the method is a simple, reliable and robust procedure for assessing the effectiveness of any separation technique of bovine X and Y spermatozoa.

Last modified: 2008-05-14 16:46:46

Applications

• Improve economics and management of dairy and beef breeder operations

Benefits

• No flow-cytometer needed
• No experienced biologist needed
• Quicker results than PCR method
• FISH is the method of choice
• Use of both paints avoids false negative results
• Robust, reproducible and easy to use
• Protocol less than 1 hour before overnight incubation
• Close agreement between FISH and flow cytometry
• Differentiate between 50%, 70% and 90% for sex-specific spermatozoa
• Useful for monitoring X-Y sperm separation methods in Bostaurus and other Bovidae

Protocol Listings

Last modified: 2009-08-25 15:58:13

Sperm - Bovine X-Y Sex Test (X-Biotin/Y-Cy3)

Hybridisation and detection protocols

Last modified: 2010-03-02 10:32:12