Star*FISH© Mouse Chromosome-Specific Probes ~ FITC Labelled
Concentrated Format

Supplier: Cambio

Conc Mouse WCP FITC Chromosome 1

Conc Mouse WCP FITC Chromosome 2

Conc Mouse WCP FITC Chromosome 3

Conc Mouse WCP FITC Chromosome 4

Conc Mouse WCP FITC Chromosome 5

Conc Mouse WCP FITC Chromosome 6

Conc Mouse WCP FITC Chromosome 7

Conc Mouse WCP FITC Chromosome 8

Conc Mouse WCP FITC Chromosome 9

Conc Mouse WCP FITC Chromosome 10

Conc Mouse WCP FITC Chromosome 11

Conc Mouse WCP FITC Chromosome 12

Conc Mouse WCP FITC Chromosome 13

Conc Mouse WCP FITC Chromosome 14

Conc Mouse WCP FITC Chromosome 15

Conc Mouse WCP FITC Chromosome 16

Conc Mouse WCP FITC Chromosome 17

Conc Mouse WCP FITC Chromosome 18

Conc Mouse WCP FITC Chromosome 19

Conc Mouse WCP FITC Chromosome X

Conc Mouse WCP FITC Chromosome Y

Detection will be required for Biotin Labelled paints

FITC Labelled paints may require amplification depending on user experience and samples.

Please note: Probes,detection kits and amplification kits are sold separately

Cambio's Star*FISH© Mouse Whole Chromosome-Specific paint systems are prepared from PCR-amplified DNA obtained from FACS-sorted chromosomes.

Mouse paints are now being used to develop model systems for studies in genetics, mutagenesis, developmental cancer biology, and as human surrogates in studying the effects of genotoxic agents. Available in this range are whole chromosome paints for use in the detection of translocation and trisomy in mice. Paints are available for chromosomes 1?19, X and Y.

Mouse probes are available labelled with biotin, FITC or Cyanine 3, and are supplied with hybridisation buffer in packs of 10 and 20 tests. Unless otherwise requested, mouse probes are supplied in the ?ready-to-use? format.

Please replace † with the number of the chromosome you wish to paint.

Protocol Listings

The FISH protocol is divided into two stages. Denaturation and Hybridisation are performed on day one. Washing and Detection are performed on day two.
The metaphase spreads are normally prepared a day in advance. On day one, the DNA of the chromosomes and paints is denatured and the Hybridisation process (reannealing) takes place overnight.

On day two the slides are washed to remove unbound DNA sequences followed by detection, counterstaining and mounting.

Day One - Denaturation & Hybridisation

Star*FISH© Mouse Chromosome-Specific Probes ~ FITC Labelled Concentrated Format

Supplier: Cambio

Conc Mouse WCP FITC Chromosome 1

Conc Mouse WCP FITC Chromosome 2

Conc Mouse WCP FITC Chromosome 3

Conc Mouse WCP FITC Chromosome 4

Conc Mouse WCP FITC Chromosome 5

Conc Mouse WCP FITC Chromosome 6

Conc Mouse WCP FITC Chromosome 7

Conc Mouse WCP FITC Chromosome 8

Conc Mouse WCP FITC Chromosome 9

Conc Mouse WCP FITC Chromosome 10

Conc Mouse WCP FITC Chromosome 11

Conc Mouse WCP FITC Chromosome 12

Conc Mouse WCP FITC Chromosome 13

Conc Mouse WCP FITC Chromosome 14

Conc Mouse WCP FITC Chromosome 15

Conc Mouse WCP FITC Chromosome 16

Conc Mouse WCP FITC Chromosome 17

Conc Mouse WCP FITC Chromosome 18

Conc Mouse WCP FITC Chromosome 19

Conc Mouse WCP FITC Chromosome X

Conc Mouse WCP FITC Chromosome Y

Detection will be required for Biotin Labelled paints

FITC Labelled paints may require amplification depending on user experience and samples.

Please note: Probes,detection kits and amplification kits are sold separately

Cambio's Star*FISH© Mouse Whole Chromosome-Specific paint systems are prepared from PCR-amplified DNA obtained from FACS-sorted chromosomes.

Mouse probes are available labelled with biotin, FITC or Cyanine 3, and are supplied with hybridisation buffer in packs of 10 and 20 tests. Unless otherwise requested, mouse probes are supplied in the ?ready-to-use? format.

Please replace † with the number of the chromosome you wish to paint.

On day two the slides are washed to remove unbound DNA sequences followed by detection, counterstaining and mounting.

Day One - Denaturation & Hybridisation

Day Two - Detection

Biotin Labelled Paints:

FITC Labelled Paints:

Cy3 Labelled Paints:

Star*FISH© Mouse Chromosome-Specific Probes ~ FITC Labelled
Concentrated Format