Star*FISH© Human Whole Chromosome-Specific Probes ~ FITC Labelled
Concentrated Format
Supplier: Cambio
Conc Human WCP FITC Chromosome 1
Conc Human WCP FITC Chromosome 2
Conc Human WCP FITC Chromosome 3
Conc Human WCP FITC Chromosome 4
Conc Human WCP FITC Chromosome 5
Conc Human WCP FITC Chromosome 6
Conc Human WCP FITC Chromosome 7
Conc Human WCP FITC Chromosome 8
Conc Human WCP FITC Chromosome 9
Conc Human WCP FITC Chromosome 10
Conc Human WCP FITC Chromosome 11
Conc Human WCP FITC Chromosome 12
Conc Human WCP FITC Chromosome 13
Conc Human WCP FITC Chromosome 14
Conc Human WCP FITC Chromosome 15
Conc Human WCP FITC Chromosome 16
Conc Human WCP FITC Chromosome 17
Conc Human WCP FITC Chromosome 18
Conc Human WCP FITC Chromosome 19
Conc Human WCP FITC Chromosome 20
Conc Human WCP FITC Chromosome 21
Conc Human WCP FITC Chromosome 22
Conc Human WCP FITC Chromosome X
Conc Human WCP FITC Chromosome Y
Detection will be required for Biotin Labelled paints
FITC Labelled paints may require amplification depending on user experience and samples.
Last modified: 2009-08-25 15:54:22
Cambio's Star*FISH© system provides an excellent method for the identification of single human chromosomes, and allows the detection of translocations and insertions on metaphase chromosomes. Marker chromosomes can also be identified and complex karyotypes analysed.
Probes are available for all chromosomes. To allow users to undertake more ambitious protocols, paints are now supplied in concentrated form with separate hybridisation buffer. This allows up to three paint colours to be used per sample with no loss of signal or specificity. The individual paints come in packs of 10 and 20 tests. Stability is guaranteed for 12 months, and many paints have shown no loss of potency after 5 years if stored at -20°C.
All Cambio paint systems for whole human chromosomes are now Cot-1 free. Ground breaking work by our collaborators in the University of Cambridge has resulted in competitor DNA-free painting. Our paints have good, if not better, specificities and signal intensities than Cot-1 containing paints, provided they are used with the optimised Star*FISH© protocols and detection reagents.
ALTechnologies whole chromosome probes labelled with digoxigenin (DIG) are now available from Cambio. Please note that for chromosomes 13, 14, 15, 21 and 22 only the 'q' arm is labelled. The probe DNA is constructed from microdissected normal human metaphase chromosome DNA, then directly amplified by PCR using a degenerate primer.
Paraffin Sections
Recently, a paraffin protocol has been devised for use of chromosome paints with paraffin sections.
Last modified: 2009-08-27 23:53:03
Recommended protocol for use with this product:
Day One - Denaturation & Hybridisation
Day Two - Detection
Last modified: 2010-03-02 10:20:58
