Star*FISH© Human Multicolour FISH (M-FISH) Kit
Ready-to-Use Format
Supplier: Cambio
Human MulticolourFISH (M-FISH) Kit
MulticolourFISH (M-FISH) allows the simultaneous visualisation of all chromosomes in a different colour and consequently allows the examination of the genome in a single hybridisation. The technology is especially useful when specimen material is limited and numerous complex chromosomal rearrangements are involved. SKY and M-FISH are the two systems used to analyse multicoloured chromosomes and the systems differ only in the way they capture and analyse the images. A MulticolourFISH experiment results in two colourful images. The raw image gives the user an indication of the hybridisation quality. The pseudo-colour image shows the classification of each pixel and rearrangements are easily spotted and identified. Numerous papers show the benefits of this elegant technique.
The multicolour probe set consists of a mixture of combinatorially labelled chromosome-specific paints of all chromosomes. These paints are derived from DOP-PCR-amplified DNA from FACS-sorted chromosomes. Hybridisation with the probe set results in high-contrast fluorescent signals, which is important for correct chromosome classification by the M-FISH software. A fluorescence microscope equipped with a FITC, Cy3, Cy3.5, Cy5, Cy5.5 filter set and M-FISH capture and classification software is required.
Current production lead time for this specialised product is approximately 14 weeks
Last modified: 2009-08-25 15:54:54
Kit comes complete with:
- 1513-MFK Probe - 1 Vial - 5 tests
- 1124-BP-50 - Blocking Protein - 1ml
- 1124-DT-25 - Detergent - 1ml
- 1124-MDA-50 - MDA - 50µl
Last modified: 2008-05-14 16:46:46
For further details on detection kits and protocols please contact us.
Last modified: 2010-03-02 10:12:10
