ScriptGuard™ RNase Inhibitor

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Last modified: 2008-07-15 14:57:15

ScriptGuard™ RNase Inhibitor is your best defense against common RNases including RNase A, RNase B, and RNase C. This recombinant RNase inhibitor protein provides reliable protection of your precious RNA samples by binding strongly to RNases in a 1:1 ratio. Advanced purification protocols and extensive quality control tests ensure ScriptGuard™ RNase Inhibitor is free of unwanted contaminants that can plague other commercially available preparations of RNase inhibitors.

Unit Definition:

One unit of ScriptGuard™ RNase Inhibitor results in 50% inhibition of 5ng of RNase A. Activity is measured by the inhibition of hydrolysis of 2,3'-cyclic monophosphate by RNase A.

Storage Buffer:

ScriptGuard™ RNase Inhibitor is supplied in a 50% glycerol solution containing 50 mM Tris-HC (pH 7.5), 0.1 mM EDTA, 10 mM DTT and 100 mM NaCl.

Quality Control:

ScriptGuard™ RNase Inhibitor is free of detectable DNA exo- and endonuclease, and RNase activities.

Last modified: 2008-05-14 16:46:46

Applications

Effectively inhibits the degradation of RNA by eukaryotic RNases in a variety of applications, including cDNA synthesis, RT-PCR and in vitro transcription and translation.

Benefits

• A potent affinity for RNases (Ki>10-14 M) ensures rapid inhibition even when trace amounts of RNase are present.
• Free of detectable RNase or DNase activity and mammalian DNA.
• Does not interfere with enzymes commonly used to prepare or analyze RNA.
• Does not release bound RNase in the absence of DTT or other reducing agents.

Last modified: 2008-05-14 16:46:46

Protocols for: ScriptGuard™ RNase Inhibitor

(catalogue number SRI6325 / SRI6310K)

Please note: all protocols off site are the responsibility of the products supplier

Last modified: 2008-05-14 16:46:46