RiboMultiplier™Sense-RNA Amplification Kit

Supplier: EPICENTRE® Biotechnologies

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RiboMultiplier™ Sense-RNA Amplification Kit

Catalogue number	Pack size
RM80510	10 Reactions

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Last modified: 2010-02-03 20:36:36

The RiboMultiplier™ Sense-RNA Amplification Kit generates amplified sense-strand RNA (sRNA) from the poly(A) RNA contained in a total RNA sample. The RiboMultiplier Kit uses a unique "terminal-tagging" process that yields amplified sRNA with a greatly improved 3´/5´ ratio (reduced 3´ bias) compared to other oligo(dT)-primed RNA amplification processes.

Figure 2. Correlation of sRNA produced by the RiboMultiplier™ Sense-RNA Amplification Kit with unamplified RNA. Log2 ratios were plotted for unamplified brain/universal human RNA (Y-axis) vs. amplified brain/universal human RNA (X-axis); r = 0.92, slope = 1.02.

Input Total RNA		sRNA yield from Universal Human Reference RNA
10 ng		8.2µg
25 ng		23.4µg
100 ng		86.7µg

Table 1. The RiboMultiplier™ Sense-RNA Amplification Kit produces microgram amounts of amplified sRNA from as little as 10 ng of input total RNA.

Gene	Transcription Length (nt)	3'/5' Ratio
Gene	Transcription Length (nt)	sRNA	aRNA
GUSB	2,245	0.8	36.8
TUBA	1,706	0.9	223
ENSA	2,512	0.9	24.3
ACTB	1,792	0.3	73.5
TFRC	5,010	0.3	256
GAPDH	1,310	1.2	181
PKG1	2,338	0.4	24.3
H3F3A	1,117	1.6	121
ALB	2,215	1.5	9.8
HPRT	1,331	0.3	6.1

Table 2. 3´/5´ ratios for RiboMultiplier™ sRNA compared to amplified aRNA produced by another RNA amplification procedure that used oligo(dT)-primed cDNA synthesis.

Last modified: 2008-09-01 12:25:12

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