PowerSoil™ DNA Isolation Kit

Sample Policy

A £5 fee to cover administration costs will be charged for sample packs. If feedback is received within 6 months of purchase, this fee, will be credited back to you.

Last modified: 2008-05-14 16:46:46

The PowerSoil™ DNA Isolation Kit* is comprised of a novel and proprietary method for isolating genomic DNA from environmental samples. The kit is intended for use with environmental samples containing a high humic acid content including difficult soil types such as compost, sediment, and manure. Other more common soil types have also been used successfully with this kit. The isolated DNA has a high level of purity allowing for more successful PCR amplification of organisms from the sample. PCR analysis has been performed to detect a variety of organisms including bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae, and actinomycetes (e.g. Streptomyces).

The PowerSoil™ DNA Kit distinguishes itself from Mo Bio's UltraClean™ Soil DNA Isolation Kit with a NEW humic substance/brown color removal procedure. This new procedure is effective at removing PCR inhibitors from even the most difficult soil types.

Environmental samples are added to a bead beating tube for rapid and thorough homogenization. Cell lysis occurs by mechanical and chemical methods. Total genomic DNA is captured on a silica membrane in a spin column format. DNA is then washed and eluted from the spin column. DNA is ready for PCR analysis and other downstream applications.

*PATENT PENDING

Last modified: 2008-05-14 16:46:46

Applications

• Biodefence
• Soil-borne Pathogens
• Agriculture
• Zoology

Benefits

• More Sample Types - Processes more soil types including difficult environmental samples.
• More PCR Results - PCR inhibition was not observed in any of the samples tested.
• More Time Savings - Purifies DNA from up to 250mg of soil in 30 minutes.
• More Purity - Gives high quality DNA free of PCR inhibitors including humic substances.
• More Clarity - No visible brown colouration was seen in any of the samples tested.

Last modified: 2008-05-14 16:46:46

Protocols for: PowerSoil™ DNA Isolation Kit

(catalogue number UC-12888-100 / UC-12888-50)

Please note: all protocols off site are the responsibility of the products supplier

Last modified: 2008-05-14 16:46:46