TransforMax™ EPI300™-T1^R Chemically Competent E.coli

TransforMax™ EPI300™-T1^R Electrocompetent E.coli

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Last modified: 2010-02-03 20:36:36

Phage T1-resistant TransforMax™ EPI300™-T1^R E. coli, like the standard TransforMax EPI300 E. coli, have been specifically engineered for use with Epicentre®'s CopyControl™ Cloning Systems.

The cells contain an up-copy mutant trfA gene whose gene product is required for initiation of replication from the oriV contained on the CopyControl pCC1™ Vectors or on clones that have been retrofitted with CopyControl™ capability using the EZ-Tn5™ <oriV/KAN-2> Insertion Kit. The trfA gene is under tight control of an inducible promoter. In addition, the phage T1-resistant TransforMax™ EPI300™-T1^R E. coli are tonA for resistance to phage T1 and phage T5.

On LB-chloramphenicol plates or in LB or SOC media supplemented by chloramphenicol, CopyControl™ clones grown in the TransforMax™ EPI300™-T1^R E. coli are maintained at a single-copy number because expression of the trfA gene is repressed. Addition of the CopyControl™ Induction Solution induces expression of the trfA gene and subsequent utilisation of oriV. The result is the induction of the CopyControl™ clones to high-copy number for high yields of higher purity DNA.

Genotype

F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK Γ- rpsL nupG trfA tonA

TransforMax™ EPI300™-T1^R Electrocompetent E. coli

• Transformation efficiency of >1 X 10¹⁰ cfu/µg of pUC19

TransforMax™ EPI300™-T1^R Chemically Competent E. coli

• Transformation efficiency of >5 x 107 cfu/µg of pUC19.
• Supplied in convenient single-use 50-µl volumes.

Last modified: 2008-05-14 16:46:46

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Last modified: 2010-02-03 20:36:36

Protocols for: TransforMax™ EPI300™-T1^R Electrocompetent E.coli & TransforMax™ EPI300™-T1^R Chemically Competent E.coli

(catalogue number EC02T15 / EC02T1101 / CCT1C0210)

Please note: all protocols off site are the responsibility of the products supplier

Last modified: 2008-05-14 16:46:46