Onar®MRSA Screen

Catalogue number

Pack size

22-2025

25 tests

22-2100

100 tests

22-2250

250 tests

Onar®MRSA - Screen

Methicillin-resistant S. aureus for real-time PCR

The Onar®MRSA Screen is an in vitro test for quantitative and rapid PCR-based detection of Methicillin-resistant Staphylococcus aureus in clinical samples. Onar®MRSA Screen is a CE-marked IVD and approved for in vitro clinical diagnostics. The test system has undergone extensive programs. We highly recommend our reliable hot-start MB Taq DNA Polymerase and our our Swab DNA Extraction Kit for optimal Onar®MRSA Screen performance and most sensitive results.

Fig. A: Target MRSA Amplified curves showing quantification of Methicillin-resistant Staphylococcus aureus using different quantitives of template DNA (5x101 to 5x 105 copies). As a negative control, the template DNA was replaced with PCR-grade water. The fluorescence channel was set it F1.

Fig. B: Internal Control Signals generated by the amplification of the internal control in channel F2. No signals indicate inhibition of the PCR by the sample material.

Background

MRSA is a specific strain of the Staphylococcus aureus bacterium that has developed antibiotic resistance to all penicillins, including methicillin and narrow-spectrum β-lactamase-resistant penicillin antibiotics. MRSA can infect other tissues when normal barriers have been breached (e.g. skin). This leads to furuncles, heavy infections and can lead to a sepsis. The Methicillin-resistant Staphylococcus aureus is described as a trigger for typical hospital related infections.

Kit Components

• Primers, probes and nucleotides at optimized concentrations
• 10x reaction buffer optimized for MB Taq Polymerase
• positive control for easy result verification
• internal control to prevent false negative results by PCR inhibition
• instruction manual
• storage box

Only purified DNA and MB Taq Polymerase need to be added.

Test Principle

The Onar®MRSA Screen test system is a test for qualitative and quantitative diagnostics of clinical samples. The test can be used with any real time PCR machine suitable with ROX and FAM dyes. The supplied primer, probes and the positive control are specific for a segment of the mecA gene of the Methicillin-resistant Staphylococcus aureus genome. The target probe emits fluorescent light at 520nm. To rule out the presence of PCR inhibitors in the individual patient specimens, an internal control is included in the kit. The internal control is detected by another probe emitting light at 600nm.

Last modified: 2008-05-14 16:46:46