OmniCleave™ Endonuclease

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Last modified: 2008-07-15 14:57:15

OmniCleave™ Endonuclease is a highly purified enzyme from a recombinant E. coli strain that degrades single- and double-stranded DNA and RNA to di-, tri-, and tetranucleotides(Figure 1). OmniCleave™ Endonuclease has the same substrate specificity and yields the same products as benzonase®, an enzyme derived from Serratia marcescens.

Figure 1. Removal of nucleic acids from cell lysates using OmniCleave™ Endonuclease. Cell lysates were prepared with or without OmniCleave Endonuclease from E. coli cells expressing human lactate dehydrogenase B (LDH) and E. coli alkaline phosphatase (AP). Two microliters of each of the cell lysates were separated by electrophoresis on a 1% agarose gel and the nucleic acids detected by ethidium bromide staining. Lane M, 1-kb ladder

Unit Definition:

One unit converts 1.0 OD260 (~60µg) of salmon sperm DNA into acid-soluble nucleotides in 30 minutes at 37°C in 50mM Tris-HCl, pH 8.0 at 25°C and 1mM MgCl₂.

Storage Buffer:

50% glycerol solution containing 50mM Tris-HCl, pH 7.5 at 25°C, 0.1M NaCl, 0.1mM EDTA, 1mM dithiothreitol, and 0.1% Triton® X-100.

Quality Control:

OmniCleave™ Endonuclease is free of detectable protease activity.

Last modified: 2008-05-14 16:46:46

Applications

• Improves ease of handling and yield of protein preparations by reducing the viscosity of cell lysates due to nucleic acids
• Removes trace contamination by nucleic acids in protein preparations
• Removes host DNA from phage preparations
• Improves electrophoretic and chromatographic separation of proteins isolated from whole cell lysates

Benefits

• Digests all forms of DNA and RNA including single-stranded and double-stranded linear, circular, and supercoiled
• Active within a broad range of conditions normally used in protein purification
• Available in bulk quantities and high concentrations

Last modified: 2008-05-14 16:46:46

Protocols for: OmniCleave™ Endonuclease

(catalogue number OC7810K / OC7850K)

Please note: all protocols off site are the responsibility of the products supplier

Last modified: 2008-05-14 16:46:46

References

1. Molloy, MP. et al. (1998) Electrophoresis 19, 837.
2. Herbert, BR. et al. (1998) Electrophoresis 19, 851.

Last modified: 2008-05-14 16:46:46