Ni IDA Agarose

Ni IDA Agarose is an effective way for purification of recombinant proteins fused with a 6 x Histidine-tag. The product consists of the tridentate chelating agent, iminodiacetic acid (IDA), covalently coupled to 4% cross-linked agarose beads.

Last modified: 2008-05-14 16:46:46

Applications

• Production of antigens for in vitro diagnosis
• Production of vaccines and adjuvants
• Production of chemical intermediates
• Antigen production for generation of antibodies
• Generation of research reagents

Benefits

• Widely used system
• Simple purification procedure under either native or denaturing conditions.
• His-tag does not affect bioactivity of protein.
• Direct purification from crude bacterial lysates.
• Best purity of protein.
• pH stability of 3-13 (short term 2-14).
• Binding capacity of up to 10 mg 6xHis-tagged protein per ml.
• Resin can be regenerated for multible uses.
• Extremely cost effective.
• Generic tool for protein expression, purification and detection: no need to produce individual antibodies to each protein of interest.

Last modified: 2008-05-14 16:46:46