MonsterScript™ Reverse Transcriptase

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Last modified: 2008-07-15 14:57:15

MonsterScript™ Reverse Transcriptase is a magnesium-dependent thermostable reverse transcriptase that completely lacks RNase H activity. The enzyme is highly efficient at producing full-length cDNA from RNA templates up to or greater than 15kb.

Quality Control:

MonsterScript™ Reverse Transcriptase is free of detectable contaminating DNA exo- and endonuclease and RNase activities.

Last modified: 2008-05-14 16:46:46

Applications

First-strand cDNA synthesis for:

• cDNA cloning and cDNA library construction
• RT-PCR and Real Time RT-PCR
• Gene expression analysis
• Making labelled cDNA probes

Benefits

• Thermostable reverse transcriptase. Full activity up to 65°C enables reverse transcription through regions of high GC content or difficult secondary structure, and increased specificity when using gene-specific primers.
• Completely lacks RNase H activity (RNase H-Minus) for full length cDNA synthesis from RNA up to or greater than 15kb.
• Greater sensitivity in RT-PCR. MonsterScript Reverse Transcriptase very efficiently produces cDNA from picogram quantities of total RNA. In addition, the MonsterScript Reaction Buffer is compatible with PCR reactions so more of the RT reaction can be used in the PCR reaction.

MonsterScript™ Reverse Transcriptase produces full-length cDNA from mRNA up to or greater than 15 kb The ≈15.2-kb human guanine nucleotide exchange factor, p532, mRNA ('HGNEFp532 mRNA') was reverse transcribed using total HeLa cellular RNA and an oligo(dT)-containing primer in a standard MonsterScript reaction. Two µl of the reverse transcription reaction was used to amplify a 1.3-kb region within 68 bases of the 5'-end of the HGNEFp532 mRNA with the FailSafe™ PCR System. 1A. Detection of the PCR product demonstrates full-length reverse transcription of the HGNEFp532 mRNA by MonsterScript Reverse Transcriptase. 1B. Agarose gel analysis of the PCR reaction shows the 1.3-kb amplicon amplified from the extreme 5'-end of the HGNEFp532 mRNA.

Last modified: 2008-05-14 16:46:46

Protocols for: MonsterScript™ 1st-Strand cDNA Synthesis Kit & MonstreScript™ Reverse Transcriptase

(catalogue number MSTA5110 / MSTA5124)

Please note: all protocols off site are the responsibility of the products supplier

Last modified: 2008-05-14 16:46:46