MaxPlax™ Lambda Packaging Extract

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Last modified: 2008-07-15 14:57:15

MaxPlax™ Lambda Packaging Extract offers maximum in vitro packaging efficiencies of cos site-containing methylated or unmethylated DNA. Traditional lambda packaging extracts are derived from two complementary lysogenic E. coli strains, BHB2690 and BHB2688, as described by Hohn. MaxPlax™ Extract utilises a restriction-free E. coli K-12 strain, NM759, in place of strain BHB2690. The sonicated extract of NM759 is combined with an extract of BHB2688 to produce MaxPlax™ Extract.

Packaging of methylated and unmethylated DNA using MaxPlax™ Lambda Packaging Extracts

Each lot of MaxPlax™ Extract is tested and guaranteed to maintain a packaging efficiency of approximately 1x10⁹pfu/µg of control lambda DNA for one year from the date of purchase if stored properly. A Certificate of Analysis stating actual packaging efficiency (pfu/µg DNA) for each respective lot of MaxPlax™ Extract is provided.

Last modified: 2008-05-14 16:46:46

Applications

• Packaging of lambda, cosmid or fosmid DNA for construction of cDNA or genomic libraries

Benefits

• High efficiencies - the unique preparation of MaxPlax Extract results in packaging efficiencies of 1-3x10⁹ pfu/µg DNA
• MaxPlax™ Extract is devoid of all known restriction activities, thereby allowing cloning of methylated DNA
• Low cost - MaxPlax™ Extract is priced substantially lower than high-efficiency extracts from other suppliers

Last modified: 2008-05-14 16:46:46

Protocols for: MaxPlax™ Lambda Packaging Extracts

(catalogue number MP5105 / MP5110 / MP5120)

Please note: all protocols off site are the responsibility of the products supplier

Last modified: 2008-05-14 16:46:46

References

1. Hohn, E.G. (1979) Methods Enzymol. 68, 299.
2. Gunther, E.G. et al. (1993) Nucl. Acids Res. 21, 3903.
3. Fiandt, M. (2000) EPICENTRE Forum 7 (3), 14.

Last modified: 2008-05-14 16:46:46