MasterPure™ Yeast RNA Purification Kit

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Last modified: 2008-07-15 14:57:15

The MasterPure™ Yeast RNA Purification Kit provides all of the reagents needed to purify RNA from cell types including: Candida, Saccharomyces, Schizosaccharomyces, and filamentous fungi.
The kit utilizes a rapid desalting process to remove contaminating macromolecules, avoiding toxic organic solvents, bead-beating, and spheroplasting.

	→		Figure 1. RNA purified with the MasterPure™ Yeast RNA Purification Kit was reverse-transcribed, labelled, and hybridized to an S. cerevisiae DNA microarray. The cDNA from cells under oxidative stress was labelled with Cy5, and cDNA from control cells was labelled with Cy3.
S. cerevisiae RNA		Labeled cDNA from MasterPure RNA

Figure 2. Agilent Technologies 2100 Bioanalyzer© electrophoretogram of the S. cerevisiae RNA shown in Figure 1. RNA was purified by the MasterPure™ Yeast RNA Purification Kit and stored at -20° C for 2 months before analysis. Observe the high purity, intact RNA.

Last modified: 2008-05-14 16:46:46

Applications

• Obtain RNA suitable for cDNA synthesis and microarray gene expression analysis

Benefits

• Avoid hot acid phenol
• Faster than spheroplasting
• Higher quality than bead-beating
• No extra enzyme or equipment to purchase
• Yields 25-50 ug RNA from 1 ml of mid log S. cerevisiae

Last modified: 2008-05-14 16:46:46

Protocols for: MasterPure™ Yeast RNA Purification Kit

(catalogue number MPY03010 / MPY03100)

Please note: all protocols off site are the responsibility of the products supplier

Last modified: 2008-05-14 16:46:46

References

1. Miller, S.A. et al., (1988) Nucl. Acids Res . 16, 1215.
2. Hoffman, L. and B.W. Jarvis. (2004) EPICENTRE Forum 11 (2), 4.

Last modified: 2008-05-14 16:46:46