MasterPure™ Yeast DNA Purification Kit

Get your Epicentre® Forum - hot off the press! Click here for direct link to Forum listings

Last modified: 2008-07-15 14:57:15

The MasterPure™ Yeast DNA Purification Kit enables efficient, high-yield purification of high molecular weight DNA from yeast and other fungi. The protocol (for 1.5ml of culture) involves non-enzymatic cell lysis at 65°C, followed by removal of protein by precipitation, then nucleic acid precipitation and resuspension.(Table 1) No lyticase or proteolytic enzymes are used in the procedure. Yeast genomic DNA yields using the MasterPure™ Kit are much higher than yields obtained with other commercially available kits.(Figure 1)
The protocol can be easily adjusted for larger or smaller samples, including single yeast colonies. The recovered nucleic acid can be used directly in many applications including PCR amplification. The MasterPure™ Yeast DNA Purification Kit is available in two sizes, either 10 or 200 DNA purifications from 1.5ml of yeast culture.

Figure 1. The MasterPure™ Yeast DNA Purification Kit gives higher yields of DNA than other kits. DNA was quantitated specifically with Hoechst fluorescent dye 33258, which gives minimal fluorescence with RNA. The data represent the average DNA yields determined by fluorometry from two experiments with S. cerevisiae and C. albicans. The MasterPure Kit produced up to 17 times more DNA from C. albicans and 12 times more DNA from S. cerevisiae than other kits

Last modified: 2008-05-14 16:46:46

Applications

• Identification and typing of fungi
• Purified DNA is suitable for many molecular biology applications

Benefits

• Higher yields of yeast chromosomal DNA than with other kits
• Versatile - recover DNA from a wide variety of yeast species
• Rapid - purify DNA in less than 40 minutes
• Simple and safe - eliminates the need for enzymatic lysis, columns, and phenol
• Quality - recovered DNA has a high molecular weight (Figure 2)and is suitable for many applications

Figure 2. Yeast DNA purified using the MasterPure™ Kit has a higher molecular weight than DNA purified using other kits. 500ng of purified yeast DNAs were analyzed by pulsed field gel electrophoresis on a 1% agarose gel. The gel was stained with ethidium bromide. Lane M, lambda DNA ladder; Lane 4, T7 DNA (40 kb).

Last modified: 2008-05-14 16:46:46

Protocols for: MasterPure™ Yeast DNA Purification Kit

(catalogue number MPY80010 / MPY80200)

Please note: all protocols off site are the responsibility of the products supplier

Last modified: 2008-05-14 16:46:46

References

1. Hoffman, L. and Moan, E. (1998) EPICENTRE Forum 5 (4), 1.
2. Jin, J., Y.-K. Lee and B.L. Wickes. (2004) J. Clin Microbiol. 42: 4293-4296.

Last modified: 2008-05-14 16:46:46