Contents > Purification & Recovery of Nucleic Acids > MasterPure™ DNA Purification Kit for Blood Version II

Catalogue number
Description
Features
Protocols

MasterPure™ DNA Purification Kit for Blood Version II

Supplier: EPICENTRE® Biotechnologies

MasterPure™ DNA Purification Kit for Blood Version II

Catalogue numberPack size
MB711400400ml of whole Blood
MB7117055ml of whole Blood
MB71174040ml of whole Blood

The MasterPure™ DNA Purification Kit for blood version II can be used to isolate exceptionally pure genomic DNA, TNA or for RNA from whole mammalian blood or buffy coat. Up to 100µg of DNA can be recovered from 600µl of buffy coat in less than 30 minutes using only 1.5-ml microcentrifuge tubes. The purified DNA can be used directly as a template in amplification reactions, for digestion with restriction enzymes, or for Southern blotting. Protocols are provided for genomic DNA purification from whole blood.


Starting MaterialYield
600µl Buffy Coat87-109µl
200µl Whole Blood3-9µl
0.6µl9-27µl
0.8µl12-36µl
1µl15-45µl
2µl30-90µl
3µl45-120µl
4µl60-180µl
5µl75-225µl

Last modified: 2008-05-14 16:46:46

Applications

  • Purification of genomic DNA from whole mammalian blood or buffy coat for most molecular biology and applications such as PCR amplification

Benefits

  • High yield of genomic DNA-up to 10 µg from 325 µl of whole blood and approximately 100 µg from 600 µl of buffy coat.
  • High DNA purity - A260/280 ratios of 1.8-2.0.
  • Rapid - can be completed in less than 30 minutes.
  • Convenient - no columns.
  • Safe - no organic solvents are used.
  • Purified DNA is PCR-ready.
  • Archive quality DNA.
  • Scalable Protocol for large or small samples.
Last modified: 2008-05-14 16:46:46

Protocols for: MasterPure™ DNA Purification Kit for Blood


MasterPure™ DNA Purification Kit for Blood Protocol

(catalogue number MG71100)


Please note: all protocols off site are the responsibility of the products supplier

Last modified: 2008-05-14 16:46:46

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