Contents > Purification & Recovery of Nucleic Acids > MasterPure™ DNA Purification Kit for Blood Version II




MasterPure™ DNA Purification Kit for Blood Version II
Supplier: EPICENTRE® Biotechnologies
MasterPure™ DNA Purification Kit for Blood Version II
| Catalogue number | Pack size |
| MB711400 | 400ml of whole Blood |
| MB711705 | 5ml of whole Blood |
| MB711740 | 40ml of whole Blood |
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Last modified: 2008-07-15 14:57:15
The MasterPure™ DNA Purification Kit for blood version II can be used to isolate exceptionally pure genomic DNA, TNA or for RNA from whole mammalian blood or buffy coat. Up to 100µg of DNA can be recovered from 600µl of buffy coat in less than 30 minutes using only 1.5-ml microcentrifuge tubes. The purified DNA can be used directly as a template in amplification reactions, for digestion with restriction enzymes, or for Southern blotting. Protocols are provided for genomic DNA purification from whole blood.
| Starting Material | Yield |
|---|---|
| 600µl Buffy Coat | 87-109µl |
| 200µl Whole Blood | 3-9µl |
| 0.6µl | 9-27µl |
| 0.8µl | 12-36µl |
| 1µl | 15-45µl |
| 2µl | 30-90µl |
| 3µl | 45-120µl |
| 4µl | 60-180µl |
| 5µl | 75-225µl |
Last modified: 2008-05-14 16:46:46
Applications
- Purification of genomic DNA from whole mammalian blood or buffy coat for most molecular biology and applications such as PCR amplification
Benefits
- High yield of genomic DNA-up to 10 µg from 325 µl of whole blood and approximately 100 µg from 600 µl of buffy coat.
- High DNA purity - A260/280 ratios of 1.8-2.0.
- Rapid - can be completed in less than 30 minutes.
- Convenient - no columns.
- Safe - no organic solvents are used.
- Purified DNA is PCR-ready.
- Archive quality DNA.
- Scalable Protocol for large or small samples.
Last modified: 2008-05-14 16:46:46
Protocols for: MasterPure™ DNA Purification Kit for Blood
MasterPure™ DNA Purification Kit for Blood Protocol

