Contents > Purification & Recovery of Nucleic Acids > MasterPure™ Complete/DNA /RNA Purification Kits
MasterPure™ Complete/DNA /RNA Purification Kits
Supplier: EPICENTRE® Biotechnologies
1X DNase Buffer
| Catalogue number | Pack size |
| MBD092H | 200ml |
2X T&C Lysis Buffer
| Catalogue number | Pack size |
| MTC085H | 500ml |
DNase I, RNase-Free
| Catalogue number | Pack size |
| D9902K | 2500U @ 1U/µl |
| D9905K | 5000U @ 1U/µl |
MasterPure™ Complete DNA & RNA Purification Kit
| Catalogue number | Pack size |
| MC85200 | 200 purifications |
| MC89010 | 10 purifications |
MasterPure™ DNA Purification Kit (For isolating TNA or DNA)
| Catalogue number | Pack size |
| MCD85201 | 200 purifications |
MasterPure™ RNA Purification Kit (For isolating RNA only)
| Catalogue number | Pack size |
| MCR85102 | 100 purifications |
MPC Protein Precipitation Solution
| Catalogue number | Pack size |
| MMP03750 | 50ml |
| MMP095H | 500ml |
Precipitation Solution for Blood
| Catalogue number | Pack size |
| MPS09250 | 250ml |
Proteinase K
| Catalogue number | Pack size |
| MPRK092 | 2ml |
Red Cell Lysis Solution - includes buffers
| Catalogue number | Pack size |
| MRC0912H | 1200ml |
RNase A
| Catalogue number | Pack size |
| MRNA092 | 2ml |
TE Buffer
| Catalogue number | Pack size |
| MTE0970 | 70ml |
Tissue and Cell Lysis Solution - includes buffers
| Catalogue number | Pack size |
| MTC096H | 600ml |
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Last modified: 2008-07-15 14:57:15
The MasterPure™ Complete DNA and RNA Purification Kit, MasterPure™ DNA Purification Kit, and MasterPure™ RNA Purification Kit use a novel, patent-pending technology that enables efficient purification of intact, high molecular weight DNA or RNA from a wide range of biological materials. Total nucleic acid (TNA), DNA, or RNA can be purified from bacteria, tissue culture cells, frozen or fresh mammalian tissue, paraffin-embedded tissue sections, mouse tail snips, and other biological materials. The protocol for TNA purification involves (1) an enzyme/salt/detergent-mediated cell lysis, (2) protein removal by precipitation, and (3) nucleic acid precipitation and resuspension. To isolate purified DNA, the sample is treated with RNase A; to isolate purified RNA, the sample is treated with RNase-Free DNase I. All manipulations are performed in standard microcentrifuge tubes. The recovered nucleic acids have an A260/280 ratio of 1.8-2.0, and can be used directly in PCR amplification (DNA and TNA) or RT-PCR (RNA). The standard MasterPure™ Complete DNA and RNA Purification Kit contains sufficient reagents for 200 DNA purifications or 100 DNA and 100 RNA purifications.
| Targets | Samples Extracted |
|---|---|
| Human & Mammalian | Serum |
| RNA & Genomic DNA | Plasma |
| HIV | Whole blood |
| E. coli | Guthrie cards |
| HCV | Buccal cells |
| B. pertussis | Liver |
| RSV | Mouse tail |
| Yeast | Kidney |
| M. tuberculosis | Saliva |
| Enterovirus | Urine |
| HPV | Sputum |
| Soy (tofu) | Tissue culture cell lines |
| Maize | Cervical cells |
| Insect tissues | Paraffin-embedded tissues |
| Streptococcus mutans |
Table 1. Examples of targets and samples analyzed using MasterPure™ DNA and RNA Purification Kits. Using an extremely simple salt precipitation protocol, TNA, DNA, or RNA can be isolated from virtually any source in less than 1 hour.
Figure 1. DNA, RNA, and total nucleic acid (TNA) purified from diverse cell sources using the MasterPure™ Complete Kit. M = kb ladder.
Figure 2. Basic MasterPure™ Complete Kit extraction protocol.
| Sample | Sample Size | TNA µg | DNAµg | RNA µg |
|---|---|---|---|---|
| HeLa/HL60 cells | 1 X 106 cells | 10-30 | 3-12 | 7-15 |
| Tissues | ||||
| Liver | >5 mg | 33-42 | 5-10 | 13-25 |
| Brain | 5 mg | 9-13 | 6-9 | 4-11 |
| Heart | 5 mg | 6-10 | 4-7 | 4-5 |
| Kidney | 5 mg | 10-17 | 3-8 | 14-17 |
| Thymus | 5 mg | 15-30 | 6-12 | 9-18 |
| Blood | 200 µl | 3-10 | 3-9 | 0.1-0.2 |
| Buffy coat | 300 µl | 40-55 | 40-55 | 3-6 |
| Mouse tail | 0.5 cm | 25-30 | 9-11 | |
| E. coli | 3.5 x 106 cells | 2.5-2.8 | 1.3-1.6 | 1.6-1.8 |
| S. mutans | 1.5 ml | 0.9 | ||
| *Yeast | 2.2 x 106 cells | 11-18 | ||
| (S. cerevisiae) | 1.1 x 107 cells | >70-78 | ||
Table 2. Example of yields of nucleic acids obtained using MasterPure™ Complete DNA and RNA Purification Kits.
*For extraction of yeast DNA, the MasterPure Yeast DNA Purification Kit is recommended.
Figure 3. Consistent purification of DNA, total nucleic acid, and RNA. Five different genomic DNA (Lanes 1-5), total nucleic acid (Lanes 6-10), and total cellular RNA (Lanes 11-15) samples were isolated from frozen bovine liver with the MasterPure™ Complete DNA and RNA Purification Kit. M = kb ladder.
Figure 4. Comparison of PCR sensitivity using templates obtained using the MasterPure™ Complete DNA and RNA Purification Kit versus a spin column kit. PCR amplification was performed after extraction from the indicated number of E. coli cells.
Last modified: 2008-05-14 16:46:46
Applications
- PCR, RT-PCR, cloning, sequencing
- Northern and Southern blotting
- BESS mutation detection
- Restriction analysis
- Genomic library preparation
Benefits
- Versatility - recover nucleic acids from a wide variety of biological materials
- Speed - purify TNA in 30 minutes, DNA in 45 minutes, and RNA in 60 minutes
- Simple - eliminates hazardous phenol/organic extractions
- Purity - recover PCR-ready DNA and RT-PCR-ready RNA.
- Efficiency - completely recover even small amounts of nucleic acid
Last modified: 2008-05-14 16:46:46
Protocols for: MasterPure™ Purification Kits
MasterPure™ Complete DNA & RNA Purification Kit Protocol
(catalogue number MC85200 / MC89010)
MasterPure™ DNA Purification Kit Protocol
(catalogue number MCD85201)
MasterPure™ RNA Purification Kit Protocol
(catalogue number MCR85102)
Please note: all protocols off site are the responsibility of the products supplier
Last modified: 2008-05-15 09:26:33
References
- Schanke, J. and Watson, J. (1998) EPICENTRE Forum 5 (2), 12.
