MasterAmp™ Tth DNA Polymerase
25mM MgCl2
| Catalogue number | Pack size |
MasterAmp™ 10X PCR Enhancer
| Catalogue number | Pack size |
MasterAmp™ Tth DNA Polymerase
| Catalogue number | Pack size |
MasterAmp™ Tth DNA Polymerase - Enzyme Only
| Catalogue number | Pack size |
Covered by issued and pending patents.
* Epicentre's® PCR products are sold under licensing arrangements with F. Hoffmann-La Roche Ltd., Roche Molecular Systems, Inc., and Applied Biosystems.
The products containing a thermostable DNA polymerase are accompanied by a limited license to use it in the Polymerase Chain Reaction (PCR) and RT-PCR for life science research in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front license fee, either by payment to Perkin-Elmer or as purchased, i.e., an authorised thermal cycler.
Last modified: 2008-07-15 14:57:15
Includes MasterAmp™ PCR Enhancer (with betaine) for improved PCR
MasterAmp™ Tth DNA Polymerase is a thermostable DNA polymerase derived from the thermophilic bacterium Thermus thermophilus. It has optimal synthetic activity at temperatures above 70deg;C and can be used at temperatures up to 95°C, making it useful for applications such as PCR. The enzyme also has thermostable reverse transcriptase activity, which enables synthesis of cDNA from RNA templates. The high reaction temperatures possible with Tth DNA Polymerase minimize problems associated with nonspecific priming and template secondary structure. MasterAmp™ Tth DNA Polymerase is highly purified using a proprietary procedure that virtually eliminates contaminating bacterial DNA. A 10X solution of MasterAmp PCR Enhancer (with betaine) is provided with the enzyme. The MasterAmp™ PCR Enhancement Technology* substantially increases both the probability of obtaining the desired amplification product and the reaction-to-reaction consistency of PCR.
Unit Definition
One unit of MasterAmp™ Tth DNA Polymerase converts 10nmoles of dNTPs into acid-insoluble material in 30 minutes at 70°C under standard assay conditions using a DNA template.
Storage Buffer:
50% glycerol containing 50mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1 mM EDTA, 0.5% Tween 20, 0.5%NP-40, and 1 mM DTT.
Quality Control:
MasterAmp™ Tth DNA Polymerase is tested for the absence of detectable, contaminating, nonspecific DNA exo- and endonuclease and RNase activities, and is functionally tested in PCR.
Last modified: 2008-05-14 16:46:46
Covered by issued and pending patents.
* Epicentre's® PCR products are sold under licensing arrangements with F. Hoffmann-La Roche Ltd., Roche Molecular Systems, Inc., and Applied Biosystems.
The products containing a thermostable DNA polymerase are accompanied by a limited license to use it in the Polymerase Chain Reaction (PCR) and RT-PCR for life science research in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front license fee, either by payment to Perkin-Elmer or as purchased, i.e., an authorised thermal cycler.
Last modified: 2008-07-15 14:57:15
Protocols for: MasterAmp™ Tth DNA Polymerase
MasterAmp™ Tth DNA Polymerase Protocol
(catalogue number TTH72100 / TTH72250 / TTH72500 / TTH7201K / TTH7205K / TTH7225N)
MasterAmp™ 10X PCR Enhancer Protocol
(catalogue number ME81201 / ME81205 / ME81210)
Please note: all protocols off site are the responsibility of the products supplier
Last modified: 2008-05-14 16:46:46
