MasterAmp™ Taq DNA Polymerase
25mM MgCl2
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MasterAmp™ 10X PCR Enhancer
| Catalogue number | Pack size |
MasterAmp™ Taq DNA Polymerase
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MasterAmp™ Taq DNA Polymerase - Enzyme Only
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Covered by issued and pending patents.
* Epicentre's® PCR products are sold under licensing arrangements with F. Hoffmann-La Roche Ltd., Roche Molecular Systems, Inc., and Applied Biosystems.
The products containing a thermostable DNA polymerase are accompanied by a limited license to use it in the Polymerase Chain Reaction (PCR) and RT-PCR for life science research in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front license fee, either by payment to Perkin-Elmer or as purchased, i.e., an authorised thermal cycler.
Last modified: 2008-07-15 14:57:15
Includes MasterAmp™ PCR Enhancer (with betaine) for improved PCR
MasterAmp™ Taq DNA Polymerase is a thermostable DNA polymerase derived from Thermus aquaticus. Having optimal activity at temperatures above 70°C, it was the first thermostable enzyme used for PCR and remains the predominant enzyme for that purpose. The enzyme has an intrinsic 5'→ 3' structure-dependent exonuclease activity, but lacks 3'→ 5' proofreading exonuclease activity. MasterAmp™ Taq DNA Polymerase is highly purified using a proprietary procedure that virtually eliminates contaminating bacterial DNA. The enzyme is provided with a 10X solution of MasterAmp PCR Enhancer (with betaine). The MasterAmp™ PCR Enhancement Technology* substantially increases both the probability of obtaining the desired amplification product and the reproducibility of PCR. It also greatly improves the consistency of PCR product yields in multiplex PCR. Different concentrations of individual or premixed PCR-tested solutions of deoxyribonucleoside 5'-triphosphates (dNTPs) are also available separately. Alternatively, dNTPs and other components are available in the MasterAmp™ Taq PCR Core Kit.
Unit Definition:
One unit of MasterAmp™ Taq DNA Polymerase converts 10nmoles of dNTPs into acid-insoluble material in 30 minutes at 70°C under standard assay conditions.
Storage Buffer:
50% glycerol containing 50 mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 0.5% Tween 20, 0.5% NP-40, and 1mM DTT.
Optimisation of [Mg2+]:
Mg2+ is required for activity of MasterAmp™ Taq DNA Polymerase. We recommend that initial experiments be performed at 1.5 mM MgCl2. The Mg2+ concentration may have to be adjusted for some template/primer pair combinations, or for dNTP concentrations other than 200µM each. Alternatively, we recommend the MasterAmp PCR Optimisation Kit without ammonium sulphate.
Quality Control:
MasterAmp™ Taq DNA Polymerase is tested for the absence of detectable contaminating nonspecific DNA exo- and endonuclease and RNase activities, and is functionally tested in PCR.
Last modified: 2008-05-14 16:46:46
Applications
- PCR amplification of DNA templates
- Multiplex PCR
Benefits
- Reliable activity, specificity, and reproducibility in PCR in conjunction with the MasterAmp™ PCR Enhancement Technology
Last modified: 2008-05-14 16:46:46
Protocols for: MasterAmp™ Taq DNA Polymerase
MasterAmp™ Taq DNA Polymerase Protocol
(catalogue number Q82100 / Q82250 / Q82500 / Q3201K / Q82250N)
MasterAmp™ Taq PCR Core Kit Protocol
(catalogue number MCQ74200)
Please note: all protocols off site are the responsibility of the products supplier
Last modified: 2008-05-14 16:46:46
