MasterAmp™ GREEN Real-Time RT-PCR Kit
MasterAmp™ Real-Time RT-PCR Kit
| Catalogue number | Pack size |
Last modified: 2008-07-15 14:57:15
The MasterAmp™ Real-Time RT-PCR Kit provides all the necessary components to perform high-sensitivity one-step or two-step quantitative RT-PCR. The kit includes a ready-to-use reaction mix containing SYBR® Green 1 dye, buffer, dNTPs, and MgCl2. In addition, the kit uses RetroAmp™ RT DNA Polymerase, which is both a thermostable DNA polymerase and has reverse transcription activity. RetroAmp™ Polymerase allows for higher RT temperatures (up to 70°C), significantly increasing the specificity and sensitivity of the RT reactions while decreasing secondary structure of RNA templates.
Another factor that improves the specificity and sensitivity of RT-PCR is the incorporation of Epicentre's® patented PCR Enhancer. The Enhancer reduces DNA and RNA secondary structure, eliminates sequence composition dependence of nucleic acid melting, and reduces pauses during DNA synthesis.
Last modified: 2008-05-14 16:46:46
Benefits
- Extremely high sensitivity and specificity (Figures 1 and 2)
- Easy-to-use PreMix contains all components for successful quantitative RT-PCR, including SYBR® Green I dye
- A thermostable enzyme increases sensitivity and specificity by reducing RNA secondary structure
- MasterAmp PCR Enhancer increases sensitivity and specificity by reducing polymerase pausing and stops during reverse transcription and PCR
- SYBR® Green I dye for detection saves time and expense compared to use of labeled primers
- The kit uses flexible protocols for real-time detection on almost all real-time PCR thermocyclers
 | Figure 1. The MasterAmp™ GREEN Real-Time RT-PCR Kit provides high-sensitivity detection.Real-time amplification of GAPDH RNA was performed using 100 ng (red), 10 ng (green), 1 ng (blue), 100 pg (pink), and 10 pg (black) total cellular RNA |
 | Figure 2. MasterAmp™ PCR Enhancer increases sensitivity and specificity of real-time RT-PCR. The products of real-time RT-PCR of a 132-bp region of β-actin gene amplified from total human placental RNA using the MasterAmp™ GREEN Real-Time RT-PCR Kit were analyzed on a gel. Results are shown using increasing amounts of MasterAmp PCR Enhancer- Lane M, 100-bp ladder;
- Lane 1, no added Enhancer;
- Lane 2, 0.5X Enhancer;
- Lane 3, 2X Enhancer.
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Last modified: 2008-05-14 16:46:46
Protocols for: MasterAmp™ GREEN Real-Time RT-PCR Kit
MasterAmp™ GREEN Real-Time RT-PCR Kit Protocol
(catalogue number MAR03100 / MAR03825)
Please note: all protocols off site are the responsibility of the products supplier
Last modified: 2008-05-14 16:46:46
