MasterAmp™ AmpliTherm™ DNA Polymerase
25mM MgCl2
| Catalogue number | Pack size |
MasterAmp™ 10X PCR Enhancer
| Catalogue number | Pack size |
MasterAmp™ AmpliTherm™ DNA Polymerase
| Catalogue number | Pack size |
MasterAmp™ AmpliTherm™ DNA Polymerase - Enzyme Only
| Catalogue number | Pack size |
Covered by issued and pending patents.
* Epicentre's® PCR products are sold under licensing arrangements with F. Hoffmann-La Roche Ltd., Roche Molecular Systems, Inc., and Applied Biosystems.
The products containing a thermostable DNA polymerase are accompanied by a limited license to use it in the Polymerase Chain Reaction (PCR) and RT-PCR for life science research in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front license fee, either by payment to Perkin-Elmer or as purchased, i.e., an authorised thermal cycler.
Last modified: 2008-07-15 14:57:15
Includes MasterAmp™ PCR Enhancer (with betaine) for improved PCR
MasterAmp™ AmpliTherm DNA Polymerase is a proprietary thermostable DNA polymerase for use in PCR. It has optimal DNA synthetic activity at temperatures above 70°C and can be used at temperatures up to 95°C. It lacks both the 5'→ 3' structure-dependent exonuclease activity like that found in Taq DNA polymerase and the 3'→ 5' proofreading exonuclease activity found in some other DNA polymerases. The enzyme is purified to homogeneity using a proprietary procedure that virtually eliminates contaminating bacterial DNA. MasterAmp™ AmpliTherm DNA Polymerase is provided with a 10X solution of MasterAmp™ PCR Enhancer (with betaine). The MasterAmp™ PCR Enhancement Technology* increases the probability of obtaining the desired amplification product, the reproducibility of PCR, and improves the consistency of PCR product yields in multiplex PCR.
Unit Definition:
One unit of MasterAmp™ AmpliTherm DNA Polymerase converts 10nmoles of dNTPs into acid-insoluble material in 30 minutes at 70°C under standard assay conditions.
Storage Buffer:
50% glycerol solution containing 50mM Tris-HCl, pH 7.5, 100mM NaCl, 0.1mM EDTA, 0.5% Tween 20, 0.5% NP-40, and 1mM DTT.
Optimisation of [Mg2+]:
Mg2+ is required for activity of MasterAmp™ AmpliTherm DNA Polymerase. We recommend that initial experiments be performed at 1.5mM MgCl2. The Mg2+ concentration may have to be adjusted for some template/primer pair combinations, or for dNTP concentrations other than 200µM each. Alternatively, we recommend the MasterAmp™ PCR Optimisation Kit without ammonium sulphate.
Quality Control:
MasterAmp™ AmpliTherm DNA Polymerase is tested for the absence of detectable contaminating exo- and endonuclease and RNase activities, and is functionally tested in PCR.
Last modified: 2008-05-14 16:46:46
Applications
- PCR amplification of DNA templates
- Multiplex PCR
Last modified: 2008-05-14 16:46:46
Protocols for: MasterAmp™ AmpliTherm™ Polymerase
MasterAmp™ AmpliTherm™ DNA Polymerase Protocol
(catalogue number AT72250 / AT72250N)
MasterAmp™ 10X PCR Enhancer Protocol
(catalogue number ME81201 / ME81205 / ME81210)
Please note: all protocols off site are the responsibility of the products supplier
Last modified: 2008-05-14 16:46:46
