Exo-Minus Klenow DNA Polymerase

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Last modified: 2008-07-15 14:57:15

Klenow DNA Polymerase Exo^- is a DNA-dependent DNA polymerase that lacks both the 5'- 3' and 3'- 5' exonuclease activities of E. coli DNA Polymerase I from which it is derived.

Concentration and Specific Activity

20 Units /µl at greater than 1 X 10⁴ Units/mg

Unit Definition

One Unit of Klenow DNA Polymerase Exo^- converts 10 nmoles of dNTPs into acid-insoluble material in 30 minutes at 37°C.

Quality Control

Klenow DNA Polymerase Exo^- is free of detectable endo- and exonuclease and RNase activities.

Last modified: 2008-05-14 16:46:46

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Last modified: 2008-07-15 14:57:15

Protocols for: Klenow DNA Polymerase Fragment Exo^-

(catalogue number KL04011K / KL06041K)

Please note: all protocols off site are the responsibility of the products supplier

Last modified: 2008-05-14 16:46:46

References

1. Feinberg, A.P. and Vogelstein, B. (1984) Anal. Biochem. 137, 266.
2. Sanger, F. et. al. (1977) Proc. Natl. Acad. Sci. USA 74, 5463.
3. Molecular Cloning: A Laboratory Manual (1989), J. Sambrook, E.F. Fritsch and T. Maniatis, editors, Cold Spring Harbor Laboratory Press, 2nd edition, v.2, p. 8.14.
4. Walter, N.G. (1995) J. Mol. Biol. 254, 856.

Last modified: 2008-05-14 16:46:46