Klenow DNA Polymerase

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Last modified: 2008-07-15 14:57:15

Klenow DNA Polymerase is derived from E. coli DNA polymerase I. This large fragment, DNA-dependent enzyme has 5'→ 3' polymerization and 3'→ 5' exonuclease activities, but lacks 5'→ 3' exonuclease activity. Klenow DNA polymerase blunt ends doublestranded DNA with singlestranded overhangs. The 3'→ 5' exonuclease activity removes 3' overhangs and the 5'→ 3' polymerization activity fills in 5' overhangs.

Unit Definition

One unit of enzyme converts 10nmol of dNTPs into acid-insoluble material in 30 minutes at 37°C.

Last modified: 2008-05-14 16:46:46

Applications

• Random primer labeling of DNA.
• DNA sequencing.
• Second-strand cDNA synthesis.
• Strand displacement amplification.

Last modified: 2008-05-14 16:46:46

Protocols for: Klenow Fragment DNA Polymerase

(catalogue number KP04061K)

Please note: all protocols off site are the responsibility of the products supplier

Last modified: 2008-05-14 16:46:46